This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
QED Bioscience
product type :
antibody
product name :
Anti-GRP78 Antibody
catalog :
11134
quantity :
100 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
1H11-1H7

The same clone is also sold as:
reactivity :
human, mouse, rat, bovine
product information
CATALOG NUMBER :
11134
PRODUCT NAME :
Anti-GRP78 Antibody
NAME+SKU :
Anti-GRP78 Antibody (11134)
PRODUCT _TYPE :
Antibody
DESCRIPTION :
Anti-Grp78 Mouse Monoclonal Antibody
SPECIFICITY SHORT :
Grp78
SPECIES REACTIVITY :
Human, mouse, rat, bovine, hamster, rabbit, monkey, Xenopus, fungal (Cryphonectria parasitica)
TARGET NAME :
GRP78
UNIPROT :
P20029
LOCATION :
Endoplasmic reticulum lumen , Melanosome , Cytoplasm , Cell surface. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (By similarity). Localizes to the cell surface in epithelial cells; high levels of free iron promotes cell surface localization (By similarity).
PROTEIN NAME :
Endoplasmic reticulum chaperone BiP
ALTERNATIVE NAMES :
EC 3.6.4.10, 78 kDa glucose-regulated protein, GRP-78, Binding-immunoglobulin protein, BiP, Heat shock protein 70 family protein 5, HSP70 family protein 5, Heat shock protein family A member 5, Immunoglobulin heavy chain-binding protein
GENE NAME :
Hspa5
ACCESSION NUMBER :
NP_001156906.1
CLONALITY :
Monoclonal
FORM :
Liquid
CONCENTRATION :
Lot Specific
SIZE 1 :
100 ug
DISTIRBUTOR PRICE :
242.9
SKU SIZE 1 :
11134
APPLICATIONS :
WB, ELISA
APPLICATION INSTRUCTIONS :
ELISA Immunoblotting: use at 0.5-1.0ug/ml. A band of ~78kDa is detected. Positive control: HeLa cell lysate.
HOST :
Mouse
FORMAT :
Purified
PURIFICATION :
Purified by immunoaffinity chromatography
ISOTYPE :
IgG2b
CLONE ID :
1H11-1H7
IMMUNOGEN :
His-tagged human GRP78 (accession no. NP_001156906.1)
RESEARCH AREA :
Heat Shock & Stress Proteins
FORMULATION :
PBS, pH 7.4, 0.09% sodium azide.
BUFFER :
Phosphate Buffered Saline
BUFFER PH :
pH 7.4
BUFFER ANTI-MICROBIAL :
0.09% Sodium Azide
TARGET BACKGROUND :
GRP78 is a ubiquitously expressed, 78kDa glucose-regulated protein commonly referred to as an immunoglobulin chain binding protein (BiP). Categorized as stress response proteins, BiPs play an important role in folding and assembly of nascent proteins and in scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5- nontranslated region of BiP mRNA. BiP IRES activity increases when cells are heat-stressed. GRP78 is also important in maintenance of cell homeostasis and the prevention of apoptosis. Levels of GRP78 protein are reduced in brains of Alzheimer’s disease patients.
FUNCTION :
Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed:12411443, PubMed:12475965). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (PubMed:12411443). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (By similarity). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity). Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation (By similarity). {UniProtKB:G3I8R9, UniProtKB:P11021, PubMed:12411443, PubMed:12475965}.
DILUTION INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE CONDITION :
This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.
SPECIFICITY :
This antibody recognizes human GRP78.
Image Name_1 :
qed-bioscience-anti-grp78-antibody-11134-1.jpg
Applications 1 :
WB
company information
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
headquarters: United States