Anti-Hsp27 Antibody | QED Bioscience 11120 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Hsp27 Antibody

catalog :

11120

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5D12.A12

The same clone is also sold as:

OriGene: TA326365, TA309363
StressMarq Biosciences: SMC-161
Invitrogen: MA5-45207, MA5-45205, MA5-45206

reactivity :

human

product information

CATALOG NUMBER :

11120

PRODUCT NAME :

Anti-Hsp27 Antibody

NAME+SKU :

Anti-Hsp27 Antibody (11120)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Hsp27 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Hsp27

SPECIES REACTIVITY :

Human

TARGET NAME :

Hsp27

UNIPROT :

P04792

LOCATION :

Cytoplasm , Nucleus , Cytoplasm, cytoskeleton, spindle

PROTEIN NAME :

Heat shock protein beta-1

ALTERNATIVE NAMES :

HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27

GENE NAME :

HSPB1

ACCESSION NUMBER :

NP_001531.1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

242.9

SKU SIZE 1 :

11120

APPLICATIONS :

WB, ELISA, IHC, IP

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 0.5-1ug/ml. A band of 27 kDa is detected. ELISA: use at 1ug/ml. Immunohistochemistry: use at 1- 10ug/ml. Immunoprecipitation: use at 1-10ug/ml. Positive control: HeLa cell lysate

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG2b

CLONE ID :

5D12.A12

IMMUNOGEN :

Human Hsp27

RESEARCH AREA :

Heat Shock & Stress Proteins

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

Hsp27 is an important heat shock protein found in normal and malignant human cells. The basic structure of most Hsps is a highly conserved amino acid sequence with an alpha-crystallin domain at the C-terminus and WD/EPF domain at the less conserved N- terminus. The N-terminus is essential for formation of high molecular weight oligomers. Hsp27 oligomers are formed by as many as 8-40 Hsp 27 monomers. The degree of oligomerization is associated with chaperone activity: large oligomers have high chaperone activity, whereas dimers have no chaperone activity. Hsp27 is localized in the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress where it may function to stabilize DNA and/or the nuclear membrane. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with activation of cytochrome C / Apaf-1 / dATP complex, thereby inhibiting activation of procaspase-9.

FUNCTION :

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742). {PubMed:10383393, PubMed:19166925, PubMed:20178975, PubMed:23728742}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

SPECIFICITY :

This antibody recognizes human Hsp27.