Anti-Hsp70 Antibody | QED Bioscience 11118 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Hsp70 Antibody

catalog :

11118

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5A5

The same clone is also sold as:

Novus Biologicals: NBP1-21690
Santa Cruz Biotechnology: sc-59571
Abcam: ab2787
OriGene: TA326358
StressMarq Biosciences: SMC-162
Invitrogen: MA5-45210, MA5-45208, MA5-45209, MA3-007

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

11118

PRODUCT NAME :

Anti-Hsp70 Antibody

NAME+SKU :

Anti-Hsp70 Antibody (11118)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Hsp70 clone 5A5 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Hsp70 clone 5A5

SPECIES REACTIVITY :

Human, mouse, rat, fish, avian, rabbit, amphibian, Drosophila, yeast

TARGET NAME :

Hsp70

UNIPROT :

P08107

ACCESSION NUMBER :

NP_005336.3

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

292.6

SKU SIZE 1 :

11118

APPLICATIONS :

WB, IHC, IP

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1ug/ml (ECL). A band of ~70 kDa is detected. Immunoprecipitation: use at 1ug/ml Immunohistochemistry/Immuno-fluorescence: use at 2 ug/ml User should determine optimal concentrations for their applications. Positive control: Heat-shocked HeLa cell lysate.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG1

CLONE ID :

5A5

IMMUNOGEN :

Recombinant human Hsp70 expressed in E. coli.

RESEARCH AREA :

Heat Shock & Stress Proteins

FORMULATION :

PBS, pH 7.4 and 50% glycerol.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. Hsp70 recognizes and binds to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound protein. The ability of Hsp70 to undergo cycles of binding and release from hydrophobic stretches of partially unfolded proteins is the basis for their role in a variety of intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C.

SPECIFICITY :

This antibody recognizes human, mouse, rat, fish, avian, rabbit, amphibian, Drosophila, and yeast Hsp70, Hsc70, Grp78, and, following heat shock, Hsp72.

Image Name_1 :

qed-bioscience-anti-hsp70-antibody-11118-1.png

Applications 1 :

Western Blot

Image Name_2 :

qed-bioscience-anti-hsp70-antibody-11118-2.png

Applications 2 :

ICC