Anti-Hsp25/Hsp27 Antibody | QED Bioscience 11110 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Hsp25/Hsp27 Antibody

catalog :

11110

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8A7

The same clone is also sold as:

Invitrogen: MA5-45121, MA5-45122, MA5-45123
Abcam: ab79868
Novus Biologicals: NB110-96431
Rockland Immunochemicals: 200-301-F55
StressMarq Biosciences: SMC-114

reactivity :

human, mouse, rat, dogs, bovine

product information

CATALOG NUMBER :

11110

PRODUCT NAME :

Anti-Hsp25/Hsp27 Antibody

NAME+SKU :

Anti-Hsp25/Hsp27 Antibody (11110)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Hsp25/Hsp27 clone 8A7 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Hsp25/Hsp27 clone 8A7

SPECIES REACTIVITY :

Human, mouse, rat, bovine, canine, guinea pig, hamster

TARGET NAME :

Hsp25/Hsp27

UNIPROT :

P04792

LOCATION :

Cytoplasm , Nucleus , Cytoplasm, cytoskeleton, spindle

PROTEIN NAME :

Heat shock protein beta-1

ALTERNATIVE NAMES :

HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27

GENE NAME :

HSPB1

ACCESSION NUMBER :

NP_001532.1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

271.6

SKU SIZE 1 :

11110

APPLICATIONS :

WB, IF

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 0.2ug/ml (ECL). A band of 25-27 kDa is detected. Immunofluorescence: use at 5ug/ml. Positive control: HeLa cell lysate

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

8A7

IMMUNOGEN :

Hsp27 peptide

RESEARCH AREA :

Heat Shock & Stress Proteins

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

Hsp25 is the mouse homologue of human Hsp27, a member of the small heat shock protein family comprised of proteins of ~15->30kDa. Oligomers of Hsp27 consist of as many as 8-40 Hsp27 monomers; large oligomers have high chaperone activity whereas dimers have no chaperone activity. Hsp27 is localized to the cytoplasm of unstressed cells but redistributes to the nucleus in response to stress where it may stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli, and, therefore, has been suggested as an important intermediate in second messenger- mediated signaling pathways. Hsp27 appears to be involved in cell differentiation and may play a role in termination of cell growth.

FUNCTION :

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742). {PubMed:10383393, PubMed:19166925, PubMed:20178975, PubMed:23728742}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

SPECIFICITY :

This antibody recognizes human, mouse, rat, bovine, canine, guinea pig, and hamster Hsp 25/27. Some cross- reactivity with alpha B crystallin.

Image Name_1 :

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Applications 1 :

IHC

Image Name_2 :

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Applications 2 :

IHC

Image Name_3 :

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Applications 3 :

IHC

Image Name_4 :

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Image Caption_4 :

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (11110). Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (11110) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Investigation at 11 days gestation. Courtesy of: Mike Welsh, Umich.

Applications 4 :

IHC

Image Name_5 :

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Image Caption_5 :

Western Blot analysis of Rat Lung tissue lysates showing detection of Hsp27 protein using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (11110). Load: 15 µg. Block: 5% blocking solution. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (11110) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse: HRP for 1 hour at RT.

Applications 5 :

Western Blot