Anti-HSF2 Antibody | QED Bioscience 11105 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-HSF2 Antibody

catalog :

11105

quantity :

100 ug

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

3-E2

The same clone is also sold as:

Santa Cruz Biotechnology: sc-13517, sc-13517 X
OriGene: TA326477, AM12012PU-S
Rockland Immunochemicals: 200-501-F51
Invitrogen: MA1-91597

reactivity :

human, mouse, rat, dogs, bovine

product information

CATALOG NUMBER :

11105

PRODUCT NAME :

Anti-HSF2 Antibody

NAME+SKU :

Anti-HSF2 Antibody (11105)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-HSF2 Rat Monoclonal Antibody

SPECIFICITY SHORT :

HSF2

SPECIES REACTIVITY :

Human, mouse, rat, rabbit, bovine, canine, porcine, guinea pig, hamster, sheep, monkey

TARGET NAME :

HSF2

UNIPROT :

P38533

LOCATION :

Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth and moves to the nucleus upon activation.

PROTEIN NAME :

Heat shock factor protein 2

ACCESSION NUMBER :

NP_001129036.1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

261.8

SKU SIZE 1 :

11105

APPLICATIONS :

WB, IP, IHC

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-5ug/ml. A band of ~69 kDa is detected. Immunohistochemistry / Immuno- fluorescence: use at 1-5 ug/ml. Immunoprecipitation: use 1-2ug per 100- 500ug total protein. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Heat-shocked HeLa cell lysate, K562 cell nuclear extract, human pancreatic tumor cells.

HOST :

Rat

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

3-E2

IMMUNOGEN :

Recombinant full-length mouse HSF2

RESEARCH AREA :

Heat Shock & Stress Proteins

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

Heat shock factor 1 (HSF1) is a heat shock transcription factor that activates the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. Up- regulation of expression of heat shock proteins in response to stress occurs at the level of transcription through a heat shock element and a HSF transcription factor. Amino acid sequences for most HSFs are highly conserved. A DNA binding domain is at the N-terminus, hydrophobic repeats (essential to the formation of active trimers) are adjacent to this binding domain, and another short hydrophobic repeat (necessary for suppression of trimerization) occurs toward the C-terminus. HSF2 exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form. Various experiments have suggested that HSF2 may play roles in differentiation and development.

FUNCTION :

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. HSF2 is expressed in a form that binds DNA constitutively but loses DNA binding by incubation at greater than 41 degrees C.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

SPECIFICITY :

This antibody recognizes HSF2 in heat- shocked cell lysates of human, mouse, rat, rabbit, bovine, canine, porcine, guinea pig, hamster, sheep, and monkey.

Image Name_1 :

qed-bioscience-anti-hsf2-antibody-11105-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-hsf2-antibody-11105-2.png

Applications 2 :

ICC

Image Name_3 :

qed-bioscience-anti-hsf2-antibody-11105-3.png

Applications 3 :

Western Blot