Anti-FKBP52 Antibody | QED Bioscience 11103 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-FKBP52 Antibody

catalog :

11103

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Hi52C

The same clone is also sold as:

StressMarq Biosciences: SMC-139
Invitrogen: MA5-45163, MA5-45165, MA5-45164
Novus Biologicals: NB110-96874
Rockland Immunochemicals: 200-301-F29
Abcam: ab59460
OriGene: TA326427

reactivity :

human, mouse, rat, dogs

product information

CATALOG NUMBER :

11103

PRODUCT NAME :

Anti-FKBP52 Antibody

NAME+SKU :

Anti-FKBP52 Antibody (11103)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-FKBP52 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

FKBP52

SPECIES REACTIVITY :

Human, mouse, rat, dog, hamster

TARGET NAME :

FKBP52

UNIPROT :

Q02790

LOCATION :

Cytoplasm, cytosol , Mitochondrion , Nucleus , Cytoplasm, cytoskeleton , Cell projection, axon

PROTEIN NAME :

Peptidyl-prolyl cis-trans isomerase FKBP4

ALTERNATIVE NAMES :

PPIase FKBP4, EC 5.2.1.8, 51 kDa FK506-binding protein, FKBP51, 52 kDa FK506-binding protein, 52 kDa FKBP, FKBP-52, 59 kDa immunophilin, p59, FK506-binding protein 4, FKBP-4, FKBP59, HSP-binding immunophilin, HBI, Immunophilin FKBP52, Rotamase [Cleaved into: Peptidyl-prolyl cis-trans isomerase FKBP4, N-terminally processed]

GENE NAME :

FKBP4

ACCESSION NUMBER :

NP_002005.1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

313.6

SKU SIZE 1 :

11103

APPLICATIONS :

WB, IP, IHC

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 0.5-1ug/ml. A band of ~52 kDa is detected. Immunohistochemistry: use at 4-5ug/ml Immunoprecipitation: 5ug on 10-20ul Protein A-Sepharose + 100ul sample. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: HeLa cell lysate, rat kidney and rat/mouse testes tissue extracts.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

Hi52C

IMMUNOGEN :

Recombinant full-length FKBP52 with an N-terminal polyhistidine tag.

RESEARCH AREA :

Heat Shock & Stress Proteins

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

FK506-binding proteins, FKBP51 and FKBP52, are high molecular weight immunophilins that are part of the mature glucocorticoid receptor heterocomplex. The N-terminal domains of these proteins bind FK506 and have peptidyl-prolyl isomerase activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the tetratricopeptide repeats that are involved in protein-protein interactions with Hsp90. Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptors differently and have different Hsp90-binding characteristics. In addition, FKBP51 is linked to the progesterone receptor while FKBP52 is linked to progesterone, androgen, and glucocorticoid receptors.

FUNCTION :

Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria. {PubMed:1279700, PubMed:1376003, PubMed:19945390, PubMed:21730050, PubMed:2378870}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

SPECIFICITY :

This antibody recognizes human, mouse, rat, dog, and hamster FKBP52.

Image Name_1 :

qed-bioscience-anti-fkbp52-antibody-11103-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-fkbp52-antibody-11103-2.png

Applications 2 :

IHC

Image Name_3 :

qed-bioscience-anti-fkbp52-antibody-11103-3.png

Applications 3 :

IHC

Image Name_4 :

qed-bioscience-anti-fkbp52-antibody-11103-4.png

Image Caption_4 :

Western Blot analysis of Human Cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C (11103). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody (11103) at 1.5 µg/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Applications 4 :

Western Blot

Image Name_5 :

qed-bioscience-anti-fkbp52-antibody-11103-5.png

Image Caption_5 :

Western Blot analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C (11103). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody (11103) at 1.5 µg/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Applications 5 :

Western Blot