This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
QED Bioscience
product type :
antibody
product name :
Anti-Hsp60 Antibody
catalog :
11100
quantity :
100 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
LK-1

The same clone is also sold as:
reactivity :
human, mouse, rat, dogs, chicken, bovine
product information
CATALOG NUMBER :
11100
PRODUCT NAME :
Anti-Hsp60 Antibody
NAME+SKU :
Anti-Hsp60 Antibody (11100)
PRODUCT _TYPE :
Antibody
DESCRIPTION :
Anti-Hsp60 Mouse Monoclonal Antibody
SPECIFICITY SHORT :
Hsp60
SPECIES REACTIVITY :
Human, mouse, rat, rabbit, bovine, canine, porcine, ovine, guinea pig, hamster, chicken, monkey, Xenopus, Drosophila
TARGET NAME :
Hsp60
UNIPROT :
P10809
LOCATION :
Mitochondrion matrix.
PROTEIN NAME :
60 kDa heat shock protein, mitochondrial
ALTERNATIVE NAMES :
EC 5.6.1.7, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein
GENE NAME :
HSPD1
ACCESSION NUMBER :
NP_002147.2
CLONALITY :
Monoclonal
FORM :
Liquid
CONCENTRATION :
Lot Specific
SIZE 1 :
100 ug
DISTIRBUTOR PRICE :
167.3
SKU SIZE 1 :
11100
APPLICATIONS :
WB, IP, FACS
APPLICATION INSTRUCTIONS :
Immunoblotting: use at 0.1-1ug/ml. A band of ~60 kDa is detected. Immunoprecipitation: use at 12.5ug/ml Flow cytometry: use at 10ug/ml These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Heat-shocked HeLa cell lysate.
HOST :
Mouse
FORMAT :
Purified
PURIFICATION :
Purified by immunoaffinity chromatography
ISOTYPE :
IgG1
CLONE ID :
LK-1
IMMUNOGEN :
Recombinant human Hsp60 expressed in E. coli.
RESEARCH AREA :
Heat Shock & Stress Proteins
FORMULATION :
PBS, pH 7.4.
BUFFER :
Phosphate Buffered Saline
BUFFER PH :
pH 7.4
TARGET BACKGROUND :
Hsp60 is an abundant protein synthesized constitutively in various cell types that is induced to higher concentrations after cell shock. It is present in mitochondria of many mammalian species and has highly similar counterparts in bacteria and plants (where it is localized to chloroplasts). In general, Hsp60 proteins are present in high concentrations, are induced in response to environmental stresses (such as heat shock) are homo-oligomeric structures of 7 or 14 subunits that dissociate reversibly in the presence of Mg2+ and ATP, have ATPase activity, and play a role in folding and assembly of oligomeric protein structures. Hsp60 has been linked to Alzheimer’s disease, coronary artery disease, multiple sclerosis, diabetes, and other autoimmune diseases.
FUNCTION :
Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:1346131, PubMed:11422376). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable). {PubMed:11422376, PubMed:1346131, PubMed:25918392}.
DILUTION INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE CONDITION :
This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.
SPECIFICITY :
This antibody recognizes human, mouse, rat, rabbit, bovine, canine, porcine, ovine, guinea pig, hamster, chicken, monkey, Xenopus, and Drosophila Hsp60 (60 kDa). It does not cross-react with bacterial or yeast Hsp60. The epitope recognized is within aa 383-447 of human Hsp60.
Image Name_1 :
qed-bioscience-anti-hsp60-antibody-11100-1.png
Applications 1 :
ICC
Image Name_2 :
qed-bioscience-anti-hsp60-antibody-11100-2.png
Applications 2 :
ICC
Image Name_3 :
qed-bioscience-anti-hsp60-antibody-11100-3.png
Applications 3 :
IHC
Image Name_4 :
qed-bioscience-anti-hsp60-antibody-11100-4.png
Image Caption_4 :
Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (11100). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (11100) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
Applications 4 :
IHC
Image Name_5 :
qed-bioscience-anti-hsp60-antibody-11100-5.png
Image Caption_5 :
Western Blot analysis of Human Cell line lysates showing detection of Hsp60 protein using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (11100). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (11100) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Applications 5 :
Western Blot
company information
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
headquarters: United States