Anti-Hsp70/Hsc70 Antibody | QED Bioscience 11099 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Hsp70/Hsc70 Antibody

catalog :

11099

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BB70

The same clone is also sold as:

Abcam: ab53496
StressMarq Biosciences: SMC-106

reactivity :

human, mouse, rat, dogs, chicken, bovine

product information

CATALOG NUMBER :

11099

PRODUCT NAME :

Anti-Hsp70/Hsc70 Antibody

NAME+SKU :

Anti-Hsp70/Hsc70 Antibody (11099)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Hsp70/Hsc70 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Hsp70/Hsc70

SPECIES REACTIVITY :

Human, mouse, rat, rabbit, monkey, hamster, guinea pig, dog, bovine, sheep, pig, chicken, yeast, fish, Xenopus

TARGET NAME :

Hsp70/Hsc70

UNIPROT :

P08106

PROTEIN NAME :

Heat shock 70 kDa protein

ALTERNATIVE NAMES :

HSP70

ACCESSION NUMBER :

NP_001006686.1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

205.1

SKU SIZE 1 :

11099

APPLICATIONS :

WB, IHC

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1ug/ml. Immunohistochemistry: use at 1-5ug/ml. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG2a

CLONE ID :

BB70

IMMUNOGEN :

Chicken Hsp70/Hsp90 complex.

RESEARCH AREA :

Heat Shock & Stress Proteins

FORMULATION :

PBS, pH 7.4, 0.1% sodium azide.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.1% Sodium Azide

TARGET BACKGROUND :

Members of the Hsp70 family are molecular chaperones that function in protein folding, transport, maturation and degradation by binding to nascent polypeptide chains as well as partially folded protein intermediates and preventing their aggregation and misfolding. The 70kDa heat shock cognate protein, Hsc70, is closely related biochemically and biologically to Hsp70.

FUNCTION :

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. {UniProtKB:P0DMV8}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for 1 month at 4°C or one year at -20°C. Avoid multiple freeze-thaw cycles.

SPECIFICITY :

This antibody detects 72 and 73kDa proteins corresponding to the predicted molecular masses of Hsp70 and Hsc70, respectively, on immunoblots of human, mouse, rat, rabbit, monkey, hamster, guinea pig, dog, bovine, sheep, pig, chicken yeast, fish, and Xenopus samples. This antibody recognizes the inducible and constitutive forms of Hsp70 and does not cross-react with Hsp90.

Image Name_1 :

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Applications 1 :

ICC

Image Name_2 :

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Applications 2 :

IHC

Image Name_3 :

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Applications 3 :

IHC

Image Name_4 :

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Image Caption_4 :

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (11099). Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11099) at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.

Applications 4 :

IHC

Image Name_5 :

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Image Caption_5 :

Western Blot analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (11099). Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11099) at 1:1000.

Applications 5 :

Western Blot

Image Name_6 :

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Image Caption_6 :

Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (11099). Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11099) at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.

Applications 6 :

Western Blot