Anti-Grp94 Antibody | QED Bioscience 11097 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Grp94 Antibody

catalog :

11097

quantity :

100 ug

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

9G10

The same clone is also sold as:

Invitrogen: MA5-45096, MA5-45098, MA5-45097, MA3-016
StressMarq Biosciences: SMC-105
Novus Biologicals: NB300-619
OriGene: TA326419
Rockland Immunochemicals: 200-501-F39
Santa Cruz Biotechnology: sc-32249 HRP, sc-32249, sc-32249 AC

reactivity :

human, mouse, rat, dogs, chicken, bovine

product information

CATALOG NUMBER :

11097

PRODUCT NAME :

Anti-Grp94 Antibody

NAME+SKU :

Anti-Grp94 Antibody (11097)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Grp94 Rat Monoclonal Antibody

SPECIFICITY SHORT :

Grp94

SPECIES REACTIVITY :

Human, mouse, rat, rabbit, monkey, hamster, guinea pig, dog, bovine, sheep, pig, chicken, Xenopus

TARGET NAME :

Grp94

UNIPROT :

P14625

LOCATION :

Endoplasmic reticulum lumen , Sarcoplasmic reticulum lumen , Melanosome

PROTEIN NAME :

Endoplasmin

ALTERNATIVE NAMES :

94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog

GENE NAME :

HSP90B1

ACCESSION NUMBER :

NP_003290.1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

205.1

SKU SIZE 1 :

11097

APPLICATIONS :

WB, IP, IHC

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 0.5-1ug/ml. A band of ~98 kDa is detected. Immunohistochemistry: use at 2-30ug/ml. Immunoprecipitation: 10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: HeLa cell lysate.

HOST :

Rat

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG2a

CLONE ID :

9G10

IMMUNOGEN :

Purified Grp94 isolated from chicken oviducts.

RESEARCH AREA :

Heat Shock & Stress Proteins

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

Glucose-regulated protein 94 (Grp94) is an endoplasmic reticulum luminal stress protein which belongs to the Hsp90 family of molecular chaperones. The sequence of Grp94 includes the COOH-terminal tetrapeptide Lys-Asp-Glu-Leu (KDEL) which is a signal sequence that causes proteins to be retained in the pre-Golgi compartments. Expression of Grp94 is up-regulated by stress conditions such as glucose starvation and heat shock, and it functions in intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen- presenting cells.

FUNCTION :

Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed:18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059). {UniProtKB:P08113, PubMed:18264092, PubMed:32272059}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid multiple freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes an ~98 kDa protein corresponding to the predicted molecular mass of Grp94 on SDS-PAGE immunoblots in human, mouse, rat, rabbit, monkey, hamster, guinea pig, dog, bovine, sheep, pig, chicken and Xenopus.

Image Name_1 :

qed-bioscience-anti-grp94-antibody-11097-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-grp94-antibody-11097-2.png

Applications 2 :

ICC

Image Name_3 :

qed-bioscience-anti-grp94-antibody-11097-3.png

Applications 3 :

Western Blot