Anti-Hsp70 Antibody | QED Bioscience 11096 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Hsp70 Antibody

catalog :

11096

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C92F3A-5

The same clone is also sold as:

OriGene: TA309388, TA326356
Novus Biologicals: NB110-61582
Abcam: ab47455, ab61907
Rockland Immunochemicals: 200-301-A27
Santa Cruz Biotechnology: sc-66048
Invitrogen: MA5-45090, MA1-10889, MA5-45092, MA5-45091
StressMarq Biosciences: SMC-100

reactivity :

human, mouse, rat, bovine

product information

CATALOG NUMBER :

11096

PRODUCT NAME :

Anti-Hsp70 Antibody

NAME+SKU :

Anti-Hsp70 Antibody (11096)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Hsp70 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Hsp70

SPECIES REACTIVITY :

Human, mouse, rat, rabbit, monkey, guinea pig, hamster, chicken, bovine, canine, ovine, porcine, fish, Drosophila, C. elegans

TARGET NAME :

Hsp70

UNIPROT :

P08107

ACCESSION NUMBER :

NP_005336.3

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

205.1

SKU SIZE 1 :

11096

APPLICATIONS :

WB, IP, IHC, ELISA, FACS

APPLICATION INSTRUCTIONS :

Immunoblotting: 1ug/ml. A band of 70kDa is detected. Immunoblotting of Hsp70 in cell lysates from human cancer cell lines with #11096. Immunohistochemistry: 1-10ug/ml Immunohistochemical staining of inflammatory cells in human colon tissue with #11096. Immunoprecipitation: 1-10ug/ml ELISA: 1-10ug/ml Flow cytometry: 10ug/ml These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: HeLa cell lysate.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

C92F3A-5

IMMUNOGEN :

Human Hsp70 purified from HeLa cells.

RESEARCH AREA :

Heat Shock & Stress Proteins

FORMULATION :

PBS containing 0.1% sodium azide

BUFFER :

Phosphate Buffered Saline

BUFFER ANTI-MICROBIAL :

0.1% Sodium Azide

TARGET BACKGROUND :

Hsp70 genes, members of a multigene family, encode heat-inducible 70kDa heat- shock proteins that function as molecular chaperones. They have been identified in most organelles of eukaryotic cells as well as in bacteria. The N-terminus of Hsp70 binds ATP with high affinity and the C- terminus binds proteins and polypeptides. Hsp70s bind nascent polypeptides as well as partially folded intermediates of proteins, thus preventing their aggregation and/or misfolding. Binding of ATP triggers a conformational change that results in the release of bound protein. Hsp70s function in protein synthesis, protein folding and oligomerization, and protein transport.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at 4C. Precipitation may occur upon freezing and thawing.

SPECIFICITY :

This antibody recognizes Hsp70 of human, mouse, rat, rabbit, monkey, guinea pig, hamster, chicken, bovine, canine, ovine, porcine, fish, Drosophila, and C. elegans and does not cross-react with Hsc70. The epitope is in the region of amino acids 436- 503 of human Hsp70.

Image Name_1 :

qed-bioscience-anti-hsp70-antibody-11096-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-hsp70-antibody-11096-2.png

Applications 2 :

IHC

Image Name_3 :

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Applications 3 :

IHC

Image Name_4 :

qed-bioscience-anti-hsp70-antibody-11096-4.png

Image Caption_4 :

Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5 (11096). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11096) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Applications 4 :

Western Blot

Image Name_5 :

qed-bioscience-anti-hsp70-antibody-11096-5.png

Image Caption_5 :

Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92F3A-5 (11096). Tissue: Heat Shocked CD3+ CD8+ T cells . Species: Mouse. Primary Antibody: Mouse Anti-Hsp70: FITC Monoclonal Antibody (11096) at 1:1000. Courtesy of: Cheryl Cameron, Vaccine and Gene Therapy Instit. Florida.

Applications 5 :

FACS