Anti-Alpha B Crystallin Antibody | QED Bioscience 11079 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Alpha B Crystallin Antibody

catalog :

11079

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1A7.D5

The same clone is also sold as:

Invitrogen: MA5-45198, MA5-45200, MA5-45201, MA5-45199, MA5-27708
StressMarq Biosciences: SMC-159
Rockland Immunochemicals: 200-301-E85
OriGene: TA326428

reactivity :

human, bovine

product information

CATALOG NUMBER :

11079

PRODUCT NAME :

Anti-Alpha B Crystallin Antibody

NAME+SKU :

Anti-Alpha B Crystallin Antibody (11079)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Alpha B Crystallin Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Alpha B Crystallin

SPECIES REACTIVITY :

Human, bovine

TARGET NAME :

Alpha B Crystallin

UNIPROT :

P02511

LOCATION :

Cytoplasm , Nucleus , Secreted , Lysosome

PROTEIN NAME :

Alpha-crystallin B chain

ALTERNATIVE NAMES :

Alpha(B-crystallin, Heat shock protein beta-5, HspB5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component

GENE NAME :

CRYAB

ACCESSION NUMBER :

NP_001276736

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

264.6

SKU SIZE 1 :

11079

APPLICATIONS :

WB, ELISA

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 0.5-1ug/ml. A band of ~20-21 kDa is detected. ELISA: use at 1ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Purified alpha B crystallin.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

1A7.D5

IMMUNOGEN :

Native alpha B crystallin

RESEARCH AREA :

Heat Shock & Stress Proteins

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

Alpha crystallins are water-soluble lens proteins of the vertebrate eye that are related to the small heat shock protein family. Lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are further divided into acidic (Alpha A) and basic (Alpha B) groups. In the lens, alpha crystallin maintains proper refractive index, however it can also function as a molecular chaperone that binds to denatured proteins, keeping them in solution and maintaining the translucency of the lens. In response to cellular stress, alpha crystallin is phosphorlyated and may serve a structural control function and play a role in protein maintenance. Both alpha A and alpha B crystallin prevent apoptosis by inhibiting caspases. Alpha B crystallin is found in many cells and organs outside the lens and is over- expressed in cells subjected to stress conditions and in several neurological disorders.

FUNCTION :

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity). {UniProtKB:P23927}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for 1 month at 4°C or one year at -20°C. Avoid multiple freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes human and bovine alpha B crystallin. It does not cross-react with alpha A crystallin, beta- L crystallin, beta-H crystallin, gamma crystallin, Hsp25, Hsp27, or Hsp47 proteins.