Anti-Alpha A Crystallin Antibody | QED Bioscience 11075 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Alpha A Crystallin Antibody

catalog :

11075

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1H3.B8

The same clone is also sold as:

StressMarq Biosciences: SMC-142
Rockland Immunochemicals: 200-301-E84
Invitrogen: MA5-27582, MA5-45175, MA5-45173, MA5-45172, MA5-45174
OriGene: TA326437
Novus Biologicals: NBP2-12875-200ug

reactivity :

human, mouse, rat, bovine

product information

CATALOG NUMBER :

11075

PRODUCT NAME :

Anti-Alpha A Crystallin Antibody

NAME+SKU :

Anti-Alpha A Crystallin Antibody (11075)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Alpha A Crystallin Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Alpha A Crystallin

SPECIES REACTIVITY :

Human, mouse, rat, bovine

TARGET NAME :

Alpha A Crystallin

UNIPROT :

P02489

LOCATION :

Cytoplasm , Nucleus

PROTEIN NAME :

Alpha-crystallin A chain

ALTERNATIVE NAMES :

Heat shock protein beta-4, HspB4 [Cleaved into: Alpha-crystallin A(1-172; Alpha-crystallin A(1-168; Alpha-crystallin A(1-162]

GENE NAME :

CRYAA

ACCESSION NUMBER :

NP_000385

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

264.6

SKU SIZE 1 :

11075

APPLICATIONS :

WB, ELISA

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 0.5-1ug/ml. A band of ~20 kDa is detected. Positive control: Purified alpha A crystallin. ELISA User should determine optimal concentrations for their application.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG1

CLONE ID :

1H3.B8

IMMUNOGEN :

Host Species: Mouse

RESEARCH AREA :

Heat Shock & Stress Proteins

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

Alpha crystallins are water-soluble lens proteins of the vertebrate eye that are related to the small heat shock protein family. Lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are further divided into acidic (Alpha A) and basic (Alpha B) groups. In the lens, alpha crystallin maintains proper refractive index, however it can also function as a molecular chaperone that binds to denatured proteins, keeping them in solution and maintaining the translucency of the lens. In response to cellular stress, alpha crystallin is phosphorlyated and may serve a structural control function and play a role in protein maintenance. Both alpha A and alpha B crystallin prevent apoptosis by inhibiting caspases.

FUNCTION :

Contributes to the transparency and refractive index of the lens (PubMed:18302245). In its oxidized form (absence of intramolecular disulfide bond), acts as a chaperone, preventing aggregation of various proteins under a wide range of stress conditions (PubMed:22120592, PubMed:31792453, PubMed:18199971, PubMed:19595763). Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA (PubMed:28935373). {PubMed:18199971, PubMed:19595763, PubMed:22120592, PubMed:28935373, PubMed:31792453, PubMed:18302245}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

SPECIFICITY :

This antibody recognizes human, mouse, rat, and bovine alpha A crystallin. Other species have not been tested. It does not cross-react with alpha B crystallin, beta-L crystallin, beta- H crystallin, gamma crystallin, Hsp25, Hsp27, or Hsp47.