product summary
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company name :
Proteintech Group
product type :
antibody
product name :
beta Actin
catalog :
66009-1-Ig
quantity :
150 ul
price :
99 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2D4H5
reactivity :
human, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
more info or order :
citations: 817
Published Application/Species/Sample/DilutionReference
  • western blot; rat; 1:3000; loading ...; fig 2a
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  • western blot; human; 1:2000; fig s9
Mao P, Liu J, Zhang Z, Zhang H, Liu H, Gao S, et al. Homologous recombination-dependent repair of telomeric DSBs in proliferating human cells. Nat Commun. 2016;7:12154 pubmed publisher
  • western blot; human; fig 4
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Zhang J, Chen Y, Lin J, Jia R, An T, Dong T, et al. Cyclovirobuxine D Exerts Anticancer Effects by Suppressing the EGFR-FAK-AKT/ERK1/2-Slug Signaling Pathway in Human Hepatocellular Carcinoma. DNA Cell Biol. 2020;: pubmed publisher
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Yang G, Li L, Xue F, Ma C, Zeng X, Zhao Y, et al. The Potential Role of PKA/CREB Signaling Pathway Concerned with Gastrodin Administration on Methamphetamine-Induced Conditioned Place Preference Rats and SH-SY5Y Cell Line. Neurotox Res. 2020;: pubmed publisher
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Mo Z, Lai X, Wang W, Chen H, He Z, Han R, et al. Identification and characterization of c-raf from orange-spotted grouper (Epinephelus coioides). Fish Shellfish Immunol. 2020;96:311-318 pubmed publisher
Huan X, Oumei C, Hongmei Q, Junxia Y, Xiaojiao M, Qingsong J. PDE9 inhibition promotes proliferation of neural stem cells via cGMP-PKG pathway following oxygen-glucose deprivation/reoxygenation injury in vitro. Neurochem Int. 2020;133:104630 pubmed publisher
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Zhang K, Bai Y, Xu R, Li J, Guan F. The mechanism of PDA/PEI/5-Fu coated esophageal stent material on inhibiting cancer associated pathological cells. J Biomed Mater Res A. 2020;108:814-821 pubmed publisher
Liu J, Yang B, Wang Y, Wu Y, Fan B, Zhu S, et al. Polychlorinated biphenyl quinone promotes macrophage polarization to CD163+ cells through Nrf2 signaling pathway. Environ Pollut. 2020;257:113587 pubmed publisher
Yang J, Hu S, Wang C, Song J, Chen C, Fan Y, et al. Fangchinoline derivatives induce cell cycle arrest and apoptosis in human leukemia cell lines via suppression of the PI3K/AKT and MAPK signaling pathway. Eur J Med Chem. 2020;186:111898 pubmed publisher
Li B, Zang G, Zhong W, Chen R, Zhang Y, Yang P, et al. Activation of CD137 signaling promotes neointimal formation by attenuating TET2 and transferrring from endothelial cell-derived exosomes to vascular smooth muscle cells. Biomed Pharmacother. 2020;121:109593 pubmed publisher
Su W, Shi J, Zhao Y, Yan F, Lei L, Li H. Porphyromonas gingivalis triggers inflammatory responses in periodontal ligament cells by succinate-succinate dehydrogenase-HIF-1α axis. Biochem Biophys Res Commun. 2020;522:184-190 pubmed publisher
Li X, Liu H, Yu W, Liu X, Liu C. Tandem mass tag (TMT) proteomic analysis of fetal lungs revealed differential expression of tight junction proteins in a rat model of congenital diaphragmatic hernia. Biomed Pharmacother. 2020;121:109621 pubmed publisher
Guan R, Wang J, Cai Z, Li Z, Wang L, Li Y, et al. Hydrogen sulfide attenuates cigarette smoke-induced airway remodeling by upregulating SIRT1 signaling pathway. Redox Biol. 2020;28:101356 pubmed publisher
Liang L, Wang M, Liu M, Zhao W, Wang X, Shi L, et al. Chronic toxicity of methamphetamine: Oxidative remodeling of pulmonary arteries. Toxicol In Vitro. 2020;62:104668 pubmed publisher
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Qaed E, Wang J, Almoiliqy M, Song Y, Liu W, Chu P, et al. Phosphocreatine Improves Cardiac Dysfunction by Normalizing Mitochondrial Respiratory Function through JAK2/STAT3 Signaling Pathway In Vivo and In Vitro. Oxid Med Cell Longev. 2019;2019:6521218 pubmed publisher
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Jiang H, Li R, Zhang Z, Chang C, Liu Y, Liu Z, et al. Retinoid X receptor α (RXRα)-mediated erythroid-2-related factor-2 (NRF2) inactivation contributes to N,N-dimethylformamide (DMF)-induced oxidative stress in HL-7702 and HuH6 cells. J Appl Toxicol. 2019;: pubmed publisher
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Lu B, Zhong J, Pan J, Yuan X, Ren M, Jiang L, et al. Gdf11 gene transfer prevents high fat diet-induced obesity and improves metabolic homeostasis in obese and STZ-induced diabetic mice. J Transl Med. 2019;17:422 pubmed publisher
Wang G, Li Z, Li X, Zhang C, Peng L. RASAL1 induces to downregulate the SCD1, leading to suppression of cell proliferation in colon cancer via LXRα/SREBP1c pathway. Biol Res. 2019;52:60 pubmed publisher
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Huang F, Chen J, Wang J, Zhu P, Lin W. Palmitic Acid Induces MicroRNA-221 Expression to Decrease Glucose Uptake in HepG2 Cells via the PI3K/AKT/GLUT4 Pathway. Biomed Res Int. 2019;2019:8171989 pubmed publisher
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Pan C, Liu Q, Wu X. HIF1α/miR-520a-3p/AKT1/mTOR Feedback Promotes The Proliferation And Glycolysis Of Gastric Cancer Cells. Cancer Manag Res. 2019;11:10145-10156 pubmed publisher
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Hu Y, Li Q, Pan Y, Xu L. Sal B Alleviates Myocardial Ischemic Injury by Inhibiting TLR4 and the Priming Phase of NLRP3 Inflammasome. Molecules. 2019;24: pubmed publisher
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Wang M, Li C, Shi W. Stomatin-like protein-2 confers neuroprotection effect in oxygen-glucose deprivation/reoxygenation-injured neurons by regulating AMPK/Nrf2 signalling. J Drug Target. 2019;:1-9 pubmed publisher
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Liu F, Li Z, He X, Yu H, Feng J. Ghrelin Attenuates Neuroinflammation and Demyelination in Experimental Autoimmune Encephalomyelitis Involving NLRP3 Inflammasome Signaling Pathway and Pyroptosis. Front Pharmacol. 2019;10:1320 pubmed publisher
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Wu Z, Xia X, Li H, Jiang S, Ma Z, Wang X. Tandem repeat sequence of duck circovirus serves as downstream sequence element to regulate viral gene expression. Vet Microbiol. 2019;239:108496 pubmed publisher
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Jiang J, Qi L, Lv Z, Jin S, Wei X, Shi F. Dietary Stevioside Supplementation Alleviates Lipopolysaccharide-Induced Intestinal Mucosal Damage through Anti-Inflammatory and Antioxidant Effects in Broiler Chickens. Antioxidants (Basel). 2019;8: pubmed publisher
Peng Y, Wang L, Wu L, Zhang L, Nie G, Guo M. Methylation of SLFN11 promotes gastric cancer growth and increases gastric cancer cell resistance to cisplatin. J Cancer. 2019;10:6124-6134 pubmed publisher
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Xu Q, Wu N, Li X, Guo C, Li C, Jiang B, et al. Inhibition of PTP1B blocks pancreatic cancer progression by targeting the PKM2/AMPK/mTOC1 pathway. Cell Death Dis. 2019;10:874 pubmed publisher
Tao W, Sun W, Liu L, Wang G, Xiao Z, Pei X, et al. Chitosan Oligosaccharide Attenuates Nonalcoholic Fatty Liver Disease Induced by High Fat Diet through Reducing Lipid Accumulation, Inflammation and Oxidative Stress in C57BL/6 Mice. Mar Drugs. 2019;17: pubmed publisher
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Chang H, Cheng H, Tsao A, Liu C, Tsai J. Multiple Functions of KBP in Neural Development Underlie Brain Anomalies in Goldberg-Shprintzen Syndrome. Front Mol Neurosci. 2019;12:265 pubmed publisher
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Wu J, Lu K, Zhu M, Xie X, Ding Y, Shao X, et al. miR-485 suppresses inflammation and proliferation of mesangial cells in an in vitro model of diabetic nephropathy by targeting NOX5. Biochem Biophys Res Commun. 2019;: pubmed publisher
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Chen Z, Zhou J, Wang X, Zhang Y, Lu X, Fan Y, et al. Screening candidate microR-15a- IRAK2 regulatory pairs for predicting the response to Staphylococcus aureus-induced mastitis in dairy cows. J Dairy Res. 2019;:1-7 pubmed publisher
Dana H, Bayramov K, Delibaşı N, Tahtasakal R, Bayramov R, Hamurcu Z, et al. Disregulation of Autophagy in the Transgenerational Cc2d1a Mouse Model of Autism. Neuromolecular Med. 2019;: pubmed publisher
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Wang T, Luo Y, Lv H, Wang J, Zhang Y, Pei R. Aptamer-Based Erythrocyte-Derived Mimic Vesicles Loaded with siRNA and Doxorubicin for the Targeted Treatment of Multidrug-Resistant Tumors. ACS Appl Mater Interfaces. 2019;11:45455-45466 pubmed publisher
Li J, Zhou Y, Du G, Qin X, Gao L. Integration of transcriptomics and network analysis deciphers the mechanisms of baicalein in improving learning and memory impairment in senescence-accelerated mouse prone 8 (SAMP8). Eur J Pharmacol. 2019;865:172789 pubmed publisher
Liu Y, Che G, Di Z, Sun W, Tian J, Ren M. Calycosin-7-O-β-D-glucoside attenuates myocardial ischemia-reperfusion injury by activating JAK2/STAT3 signaling pathway via the regulation of IL-10 secretion in mice. Mol Cell Biochem. 2019;: pubmed publisher
Yang L, Sun Y, Liu Y, Yin N, Bu F, Yu H, et al. PTP1B promotes macrophage activation by regulating the NF-κB pathway in alcoholic liver injury. Toxicol Lett. 2019;319:11-21 pubmed publisher
Chen S, Yi Y, Zeng D, Tang Y, Kang X, Zhang P, et al. Hydrogen Sulfide Attenuates β2-Microglobulin-Induced Cognitive Dysfunction: Involving Recovery of Hippocampal Autophagic Flux. Front Behav Neurosci. 2019;13:244 pubmed publisher
Zhang Z, Wang J, Zhu Y, Zhang H, Wang H. Astragaloside IV alleviates myocardial damage induced by type 2 diabetes via improving energy metabolism. Mol Med Rep. 2019;20:4612-4622 pubmed publisher
Qi W, Li Z, Xia L, Dai J, Zhang Q, Wu C, et al. LncRNA GABPB1-AS1 and GABPB1 regulate oxidative stress during erastin-induced ferroptosis in HepG2 hepatocellular carcinoma cells. Sci Rep. 2019;9:16185 pubmed publisher
Luo X, Wu X, Wei X, Wang A, Wang S, Wang J. LncRNA ADPGK-AS1 regulated cell proliferation, invasion, migration and apoptosis via targeting miR-542-3p in osteosarcoma. Eur Rev Med Pharmacol Sci. 2019;23:8751-8760 pubmed publisher
Luo Y, Pang X, Ansari A, Wu X, Li H, Zhang Z, et al. Visfatin Exerts Immunotherapeutic Effects in Lipopolysaccharide-Induced Acute Lung Injury in Murine Model. Inflammation. 2019;: pubmed publisher
Zhang Q, Zhao X, Zhang C, Wang W, Li F, Liu D, et al. Overexpressed PKMYT1 promotes tumor progression and associates with poor survival in esophageal squamous cell carcinoma. Cancer Manag Res. 2019;11:7813-7824 pubmed publisher
Zhu H, Liu L, Deng H, Li Z, Sheng J, He X, et al. Astrocyte elevated gene 1 (AEG-1) promotes anoikis resistance and metastasis by inducing autophagy in hepatocellular carcinoma. J Cell Physiol. 2019;: pubmed publisher
Wang S, Zhang J, Wang T, Ren F, Liu X, Lu Y, et al. Endocytic degradation of ErbB2 mediates the effectiveness of neratinib in the suppression of ErbB2-positive ovarian cancer. Int J Biochem Cell Biol. 2019;117:105640 pubmed publisher
Zhou C, Taslima F, Abdelhamid M, Kim S, Akatsu H, Michikawa M, et al. Beta-Amyloid Increases the Expression Levels of Tid1 Responsible for Neuronal Cell Death and Amyloid Beta Production. Mol Neurobiol. 2019;: pubmed publisher
Yang S, Liu B, Yin S, Shang Y, Zhang X, Khan M, et al. Porcine Circovirus Type 2 Induces Single Immunoglobulin Interleukin-1 Related Receptor (SIGIRR) Downregulation to Promote Interleukin-1β Upregulation in Porcine Alveolar Macrophage. Viruses. 2019;11: pubmed publisher
Bai Z, Liu X, Guan Q, Ding N, Wei Q, Tong B, et al. 5-(3,4,5-trimethoxybenzoyl)-4-methyl-2-(p-tolyl) imidazol (BZML) targets tubulin and DNA to induce anticancer activity and overcome multidrug resistance in colorectal cancer cells. Chem Biol Interact. 2019;315:108886 pubmed publisher
Ye C, Wang D, Liu H, Ma H, Dong Y, Yao M, et al. An Improved Enzyme-Linked Focus Formation Assay Revealed Baloxavir Acid as a Potential Antiviral Therapeutic Against Hantavirus Infection. Front Pharmacol. 2019;10:1203 pubmed publisher
Li Y, Yan G, Zhang J, Chen W, Ding T, Yin Y, et al. LncRNA HOXA11-AS regulates calcium oxalate crystal-induced renal inflammation via miR-124-3p/MCP-1. J Cell Mol Med. 2019;: pubmed publisher
Yang Y, Sun Q, Xu X, Yang X, Gao Y, Sun X, et al. Oral Administration of Succinoglycan Riclin Improves Diet-Induced Hypercholesterolemia in Mice. J Agric Food Chem. 2019;67:13307-13317 pubmed publisher
Liu J, Hu Y, Feng Y, Guo Y, Liu Y, Yang J, et al. Rafoxanide promotes apoptosis and autophagy of gastric cancer cells by suppressing PI3K /Akt/mTOR pathway. Exp Cell Res. 2019;385:111691 pubmed publisher
Huo X, Ji L, Zhang Y, Lv P, Cao X, Wang Q, et al. The Nuclear Matrix Protein SAFB Cooperates with Major Satellite RNAs to Stabilize Heterochromatin Architecture Partially through Phase Separation. Mol Cell. 2019;: pubmed publisher
Jiang Y, Liu J, Lin J, Li S, Xu Y, Min Q, et al. K562 cell-derived exosomes suppress the adhesive function of bone marrow mesenchymal stem cells via delivery of miR-711. Biochem Biophys Res Commun. 2019;: pubmed publisher
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image
image 1 :
Proteintech Group 66009-1-Ig image 1
The beta Actin antibody from Proteintech is a mouse monoclonal antibody to a fusion protein of human beta Actin. This antibody recognizes human, mouse, rat, hamster, zebrafish,monkey antigen. The beta Actin antibody has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA analysis.
product information
CatalogNo :
66009-1-Ig
AntigenName :
beta Actin
Package :
150 ul
Price :
99 USD
FullName :
actin, beta
Immunogen :
Fusion Protein
Species :
broiler chick, chick, chicken, chicks, common carp, cow, drosophila, goat, grouper, hamster, human, juvenile orange-spotted grouper, monkey, mouse, muscovy duck, pig, rat, swine, zebrafish
Host :
Mouse
IsConjugated :
Unconjugated
AntigenSpecies :
human
CloneNo :
2D4H5
Application :
WB, IP, IHC, IF, FC, ELISA
Clonlity :
Monoclonal
IsoType :
IgG2b
Synonyms :
ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin
PrimaryOrSecondary :
Primary
AntibodyBuffer :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
GenBankNo :
NM_001101
Category :
Binding Proteins;Cell Cycle;Cytoskeleton/Scaffold Proteins;Metabolism;Transport/Trafficking;
PurifyMethod :
Protein A purification
NewAb :
False
IsSellable :
True
Feature :
siRNA
AppTiter :
FC 1:0 ; IF 1:1000 ; IHC 1:500 ; IP 1:10000 ; WB 1:20000 ;
more info or order :
company information
Proteintech Group
2201 W. Campbell Park Dr. STE12
Chicago, IL 60612
Proteintech@ptglab.com
https://www.ptglab.com
1-312-455-8498
headquarters: USA
At Proteintech, we produce every single antibody we sell; we do not rely on or supply to any other antibody providers: our products are unique and we are 100% accountable for each one. We realize this accountability by validating in-house, providing extensive technical support and guaranteeing your success: in addition to helping you troubleshoot your experiment, we will offer you a full cash refund if you are in any way dissatisfied. We can guarantee satisfaction because we have confidence in our products, confidence cultivated by the science behind our antibodies: we make them using as much of the native protein as possible, and purifying them using affinity purification with the original antigen. We carry out antibody production over a 102-day period, which allows for better antigen fitting to MHC molecules and affinity maturation in the host. This approach results in higher affinity antibodies with greater sensitivity, which you can use in any application and in multiple species.
You can only buy Proteintech antibodies directly from Proteintech or via one of its approved distributors — when you receive your antibody and see the Proteintech logo on the vial, know that you hold something that is truly unique.