This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Proteintech Group
product type :
antibody
product name :
IFT20
catalog :
13615-1-AP
quantity :
150UL
price :
299 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, western blot knockout validation
citations: 31
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; fig 2
  • immunocytochemistry; mouse; 1:500; fig s5
Noda K, Kitami M, Kitami K, Kaku M, Komatsu Y. Canonical and noncanonical intraflagellar transport regulates craniofacial skeletal development. Proc Natl Acad Sci U S A. 2016;113:E2589-97 pubmed publisher
  • western blot; human; 1:1500; loading ...; fig 10c
Boukhalfa A, Nascimbeni A, Ramel D, Dupont N, Hirsch E, Gayral S, et al. PI3KC2α-dependent and VPS34-independent generation of PI3P controls primary cilium-mediated autophagy in response to shear stress. Nat Commun. 2020;11:294 pubmed publisher
  • immunocytochemistry; human; 1:300; loading ...; fig s6b
  • western blot; human; 1:1000; loading ...; fig s6c
Stoetzel C, Bär S, De Craene J, Scheidecker S, Etard C, Chicher J, et al. A mutation in VPS15 (PIK3R4) causes a ciliopathy and affects IFT20 release from the cis-Golgi. Nat Commun. 2016;7:13586 pubmed publisher
Su S, Begum S, Ezratty E. An IFT20 mechano-trafficking axis is required for integrin recycling, focal adhesion dynamics and polarized cell migration. Mol Biol Cell. 2020;:mbcE20040232 pubmed publisher
Ran J, Liu M, Feng J, Li H, Ma H, Song T, et al. ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia. Dev Cell. 2020;53:287-299.e5 pubmed publisher
Fabbri L, Dufies M, Lacas Gervais S, Gardie B, Gad Lapiteau S, Parola J, et al. Identification of a new aggressive axis driven by ciliogenesis and absence of VDAC1-ΔC in clear cell Renal Cell Carcinoma patients. Theranostics. 2020;10:2696-2713 pubmed publisher
Iaconis D, Crina C, Brillante S, Indrieri A, Morleo M, Franco B. The HOPS complex subunit VPS39 controls ciliogenesis through autophagy. Hum Mol Genet. 2020;29:1018-1029 pubmed publisher
Wang Y, Huang Y, Liu J, Zhang J, Xu M, You Z, et al. Acetyltransferase GCN5 regulates autophagy and lysosome biogenesis by targeting TFEB. EMBO Rep. 2019;:e48335 pubmed publisher
Zemirli N, Boukhalfa A, Dupont N, Botti J, Codogno P, Morel E. The primary cilium protein folliculin is part of the autophagy signaling pathway to regulate epithelial cell size in response to fluid flow. Cell Stress. 2019;3:100-109 pubmed publisher
Finetti F, Cassioli C, Cianfanelli V, Onnis A, Paccagnini E, Kabanova A, et al. The intraflagellar transport protein IFT20 controls lysosome biogenesis by regulating the post-Golgi transport of acid hydrolases. Cell Death Differ. 2019;: pubmed publisher
Liu W, He X, Yang S, Zouari R, Wang J, Wu H, et al. Bi-allelic Mutations in TTC21A Induce Asthenoteratospermia in Humans and Mice. Am J Hum Genet. 2019;104:738-748 pubmed publisher
Wehrle A, Witkos T, Unger S, Schneider J, Follit J, Hermann J, et al. Hypomorphic mutations of TRIP11 cause odontochondrodysplasia. JCI Insight. 2019;4: pubmed publisher
Tu H, Qin X, Liu Z, Song Z, Hu H, Zhang Y, et al. Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis. Nat Commun. 2018;9:5277 pubmed publisher
Vuolo L, Stevenson N, Heesom K, Stephens D. Dynein-2 intermediate chains play crucial but distinct roles in primary cilia formation and function. elife. 2018;7: pubmed publisher
Galati D, Sullivan K, Pham A, Espinosa J, Pearson C. Trisomy 21 Represses Cilia Formation and Function. Dev Cell. 2018;46:641-650.e6 pubmed publisher
Kasahara K, Aoki H, Kiyono T, Wang S, Kagiwada H, Yuge M, et al. EGF receptor kinase suppresses ciliogenesis through activation of USP8 deubiquitinase. Nat Commun. 2018;9:758 pubmed publisher
Han S, Jung J, Im S, Lee S, Jang B, Park K, et al. Deficiency of primary cilia in kidney epithelial cells induces epithelial to mesenchymal transition. Biochem Biophys Res Commun. 2018;496:450-454 pubmed publisher
Schmid F, Schou K, Vilhelm M, Holm M, Breslin L, Farinelli P, et al. IFT20 modulates ciliary PDGFRα signaling by regulating the stability of Cbl E3 ubiquitin ligases. J Cell Biol. 2018;217:151-161 pubmed publisher
Lee S, Joo K, Jung E, Hong H, Seo J, Kim J. Export of membrane proteins from the Golgi complex to the primary cilium requires the kinesin motor, KIFC1. FASEB J. 2018;32:957-968 pubmed publisher
Martin L, Kaci N, Estibals V, Goudin N, Garfa Traoré M, Benoist Lasselin C, et al. Constitutively-active FGFR3 disrupts primary cilium length and IFT20 trafficking in various chondrocyte models of achondroplasia. Hum Mol Genet. 2018;27:1-13 pubmed publisher
Guen V, Chavarria T, Kröger C, Ye X, Weinberg R, Lees J. EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling. Proc Natl Acad Sci U S A. 2017;114:E10532-E10539 pubmed publisher
Kurtenbach S, Gießl A, Strömberg S, Kremers J, Atorf J, Rasche S, et al. The BEACH Protein LRBA Promotes the Localization of the Heterotrimeric G-protein Golf to Olfactory Cilia. Sci Rep. 2017;7:8409 pubmed publisher
Katoh Y, Michisaka S, Nozaki S, Funabashi T, Hirano T, Takei R, et al. Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated, homology-independent knock-in system. Mol Biol Cell. 2017;28:898-906 pubmed publisher
Nishita M, Park S, Nishio T, Kamizaki K, Wang Z, Tamada K, et al. Ror2 signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness. Sci Rep. 2017;7:1 pubmed publisher
Fu W, Wang L, Kim S, Li J, Dynlacht B. Role for the IFT-A Complex in Selective Transport to the Primary Cilium. Cell Rep. 2016;17:1505-1517 pubmed publisher
Inaba H, Goto H, Kasahara K, Kumamoto K, Yonemura S, Inoko A, et al. Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway. J Cell Biol. 2016;212:409-23 pubmed publisher
Vivar O, Masi G, Carpier J, Magalhaes J, Galgano D, Pazour G, et al. IFT20 controls LAT recruitment to the immune synapse and T-cell activation in vivo. Proc Natl Acad Sci U S A. 2016;113:386-91 pubmed publisher
Shin J, Bae D, Kim E, Kim H, Park S, Jo Y, et al. Autophagy Regulates Formation of Primary Cilia in Mefloquine-Treated Cells. Biomol Ther (Seoul). 2015;23:327-32 pubmed publisher
Liu F, Chen J, Yu S, Raghupathy R, Liu X, Qin Y, et al. Knockout of RP2 decreases GRK1 and rod transducin subunits and leads to photoreceptor degeneration in zebrafish. Hum Mol Genet. 2015;24:4648-59 pubmed publisher
Shin J, Kim P, Kim E, Park S, Jo Y, Hwang J, et al. BIX-01294-induced autophagy regulates elongation of primary cilia. Biochem Biophys Res Commun. 2015;460:428-33 pubmed publisher
Lu Q, Insinna C, Ott C, Stauffer J, Pintado P, Rahajeng J, et al. Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. Nat Cell Biol. 2015;17:228-240 pubmed publisher
product information
CatalogNo :
13615-1-AP
AntigenName :
IFT20
Package :
150UL
Price :
299 USD
Exsists20ul :
20ul trial size available
FullName :
intraflagellar transport 20 homolog (Chlamydomonas)
Immunogen :
Recombinant Protein
Species :
dog, human, mouse, rat, zebrafish
Host :
Rabbit
IsConjugated :
Unconjugated
AntigenSpecies :
human
Application :
WB, IP, IHC, IF, FC, CoIP, ELISA
Clonlity :
Polyclonal
IsoType :
IgG
Synonyms :
hIFT20, IFT20
PrimaryOrSecondary :
Primary
AntibodyBuffer :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
GenBankNo :
BC038094
Category :
Binding Proteins;Ciliary Protein;Cytoskeleton/Scaffold Proteins;
PurifyMethod :
Antigen affinity purification
NewAb :
False
IsSellable :
True
Feature :
siRNA
AppTiter :
IF 1:50 ; IHC 1:50 ; IP 1:700 ; WB 1:500 ;
company information
Proteintech Group
2201 W. Campbell Park Dr. STE12
Chicago, IL 60612
Proteintech@ptglab.com
https://www.ptglab.com
1-312-455-8498
headquarters: USA
At Proteintech, we produce every single antibody we sell; we do not rely on or supply to any other antibody providers: our products are unique and we are 100% accountable for each one. We realize this accountability by validating in-house, providing extensive technical support and guaranteeing your success: in addition to helping you troubleshoot your experiment, we will offer you a full cash refund if you are in any way dissatisfied. We can guarantee satisfaction because we have confidence in our products, confidence cultivated by the science behind our antibodies: we make them using as much of the native protein as possible, and purifying them using affinity purification with the original antigen. We carry out antibody production over a 102-day period, which allows for better antigen fitting to MHC molecules and affinity maturation in the host. This approach results in higher affinity antibodies with greater sensitivity, which you can use in any application and in multiple species.
You can only buy Proteintech antibodies directly from Proteintech or via one of its approved distributors — when you receive your antibody and see the Proteintech logo on the vial, know that you hold something that is truly unique.