This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Proteintech Group
product type :
antibody
product name :
GCLC
catalog :
12601-1-AP
quantity :
150UL
price :
299 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation
citations: 35
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; loading ...; fig 1j
Wei Y, Lu M, Mei M, Wang H, Han Z, Chen M, et al. Pyridoxine induces glutathione synthesis via PKM2-mediated Nrf2 transactivation and confers neuroprotection. Nat Commun. 2020;11:941 pubmed publisher
Zhang Y, Zhao S, Fu Y, Yan L, Feng Y, Chen Y, et al. Computational repositioning of dimethyl fumarate for treating alcoholic liver disease. Cell Death Dis. 2020;11:641 pubmed publisher
Chen X, Qi J, Wu Q, Jiang H, Wang J, Chen W, et al. High glucose inhibits vascular endothelial Keap1/Nrf2/ARE signal pathway via downregulation of monomethyltransferase SET8 expression. Acta Biochim Biophys Sin (Shanghai). 2020;52:506-516 pubmed publisher
Zhao X, Shi Y, Zhang D, Tong X, Sun Y, Xue X, et al. Autophagy inducer activates Nrf2-ARE pathway to attenuate aberrant alveolarization in neonatal rats with bronchopulmonary dysplasia. Life Sci. 2020;252:117662 pubmed publisher
Qi J, Chen X, Wu Q, Wang J, Zhang H, Mao A, et al. Fasting Induces Hepatocellular Carcinoma Cell Apoptosis by Inhibiting SET8 Expression. Oxid Med Cell Longev. 2020;2020:3985089 pubmed publisher
Wang S, Cheng H, Wang L, Zhao R, Guan D. Overexpression of NRF1-742 or NRF1-772 Reduces Arsenic-Induced Cytotoxicity and Apoptosis in Human HaCaT Keratinocytes. Int J Mol Sci. 2020;21: pubmed publisher
Wang Z, Cai L, Li H, Liang M, Zhang Y, Wu Q, et al. Rice protein stimulates endogenous antioxidant response attributed to methionine availability in growing rats. J Food Biochem. 2020;44:e13180 pubmed publisher
Wang X, Liu M, Zhu M, Shi L, Liu L, Zhao Y, et al. Resveratrol protects the integrity of alveolar epithelial barrier via SIRT1/PTEN/p-Akt pathway in methamphetamine-induced chronic lung injury. Cell Prolif. 2020;53:e12773 pubmed publisher
Liang L, Wang M, Liu M, Zhao W, Wang X, Shi L, et al. Chronic toxicity of methamphetamine: Oxidative remodeling of pulmonary arteries. Toxicol In Vitro. 2020;62:104668 pubmed publisher
Lee J, Ishihara K, Notomi S, Efstathiou N, Ueta T, Maidana D, et al. Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells. Biochem Biophys Res Commun. 2019;: pubmed publisher
Shu G, Qiu Y, Hao J, Fu Q, Deng X. γ-Oryzanol alleviates acetaminophen-induced liver injury: roles of modulating AMPK/GSK3β/Nrf2 and NF-κB signaling pathways. Food Funct. 2019;10:6858-6872 pubmed publisher
Yu H, Lin T, Chen W, Cao W, Zhang C, Wang T, et al. Size and temporal-dependent efficacy of oltipraz-loaded PLGA nanoparticles for treatment of acute kidney injury and fibrosis. Biomaterials. 2019;219:119368 pubmed publisher
Lu Q, Tan S, Gu W, Li F, Hua W, Zhang S, et al. Phytochemical composition, isolation and hepatoprotective activity of active fraction from Veronica ciliata against acetaminophen-induced acute liver injury via p62-Keap1-Nrf2 signaling pathway. J Ethnopharmacol. 2019;243:112089 pubmed publisher
Wang Y, Zhang J, Ren S, Sun D, Huang H, Wang H, et al. Branched-Chain Amino Acid Metabolic Reprogramming Orchestrates Drug Resistance to EGFR Tyrosine Kinase Inhibitors. Cell Rep. 2019;28:512-525.e6 pubmed publisher
Li S, Zhou J, Xu S, Li J, Liu J, Lu Y, et al. Induction of Nrf2 pathway by Dendrobium nobile Lindl. alkaloids protects against carbon tetrachloride induced acute liver injury. Biomed Pharmacother. 2019;117:109073 pubmed publisher
Betharia S, Rondόn Ortiz A, Brown D. Disubstituted Dithiolethione ACDT Exerts Neuroprotective Effects Against 6-Hydroxydopamine-Induced Oxidative Stress in SH-SY5Y Cells. Neurochem Res. 2019;: pubmed publisher
Wang Z, Chen Z, Jiang Z, Luo P, Liu L, Huang Y, et al. Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents. Nat Commun. 2019;10:2538 pubmed publisher
Zhang J, Hong Y, Cao W, Yin S, Shi H, Ying W. SIRT2, ERK and Nrf2 Mediate NAD+ Treatment-Induced Increase in the Antioxidant Capacity of PC12 Cells Under Basal Conditions. Front Mol Neurosci. 2019;12:108 pubmed publisher
Wang Z, Liang M, Li H, Cai L, He H, Wu Q, et al. l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats. J Sci Food Agric. 2019;99:4849-4862 pubmed publisher
Peng L, Zhao Y, Li Y, Zhou Y, Li L, Lei S, et al. Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury. J Mol Med (Berl). 2019;97:189-199 pubmed publisher
Yuan X, Yang C, Wang X, Zhang L, Gao X, Shi Z. Progesterone maintains the status of granulosa cells and slows follicle development partly through PGRMC1. J Cell Physiol. 2018;234:709-720 pubmed publisher
Ning C, Gao X, Wang C, Kong Y, Liu Z, Sun H, et al. Ginsenoside Rg1 protects against acetaminophen-induced liver injury via activating Nrf2 signaling pathway in vivo and in vitro. Regul Toxicol Pharmacol. 2018;98:58-68 pubmed publisher
Wei X, Mo X, An F, Ji X, Lu Y. 2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone, a potent Nrf2/ARE pathway inhibitor, reverses drug resistance by decreasing glutathione synthesis and drug efflux in BEL-7402/5-FU cells. Food Chem Toxicol. 2018;119:252-259 pubmed publisher
Piulats J, Vidal A, García Rodríguez F, Muñoz C, Nadal M, Moutinho C, et al. Orthoxenografts of Testicular Germ Cell Tumors Demonstrate Genomic Changes Associated with Cisplatin Resistance and Identify PDMP as a Resensitizing Agent. Clin Cancer Res. 2018;24:3755-3766 pubmed publisher
Liang M, Wang Z, Li H, Cai L, Pan J, He H, et al. l-Arginine induces antioxidant response to prevent oxidative stress via stimulation of glutathione synthesis and activation of Nrf2 pathway. Food Chem Toxicol. 2018;115:315-328 pubmed publisher
Shi A, Shi H, Wang Y, Liu X, Cheng Y, Li H, et al. Activation of Nrf2 pathway and inhibition of NLRP3 inflammasome activation contribute to the protective effect of chlorogenic acid on acute liver injury. Int Immunopharmacol. 2018;54:125-130 pubmed publisher
Vu K, Hulleman J. An inducible form of Nrf2 confers enhanced protection against acute oxidative stresses in RPE cells. Exp Eye Res. 2017;164:31-36 pubmed publisher
Tomasovic A, Kurrle N, Wempe F, De Zolt S, Scheibe S, Koli K, et al. Ltbp4 regulates Pdgfrβ expression via TGFβ-dependent modulation of Nrf2 transcription factor function. Matrix Biol. 2017;59:109-120 pubmed publisher
Cao W, Hong Y, Chen H, Wu F, Wei X, Ying W. SIRT2 mediates NADH-induced increases in Nrf2, GCL, and glutathione by modulating Akt phosphorylation in PC12 cells. FEBS Lett. 2016;590:2241-55 pubmed publisher
Zheng Z, Xu H, Suo S, Xu X, Ni M, Gu L, et al. The Essential Role of H19 Contributing to Cisplatin Resistance by Regulating Glutathione Metabolism in High-Grade Serous Ovarian Cancer. Sci Rep. 2016;6:26093 pubmed publisher
Nikkanen J, Forsström S, Euro L, Paetau I, Kohnz R, Wang L, et al. Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism. Cell Metab. 2016;23:635-48 pubmed publisher
Yuan X, Fan Y, Yang C, Gao X, Zhang L, Hu Y, et al. Progesterone amplifies oxidative stress signal and promotes NO production via H2O2 in mouse kidney arterial endothelial cells. J Steroid Biochem Mol Biol. 2016;155:104-11 pubmed publisher
Truitt M, Conn C, Shi Z, Pang X, Tokuyasu T, Coady A, et al. Differential Requirements for eIF4E Dose in Normal Development and Cancer. Cell. 2015;162:59-71 pubmed publisher
Tomasovic A, Kurrle N, Sürün D, Heidler J, Husnjak K, Poser I, et al. Sestrin 2 protein regulates platelet-derived growth factor receptor β (Pdgfrβ) expression by modulating proteasomal and Nrf2 transcription factor functions. J Biol Chem. 2015;290:9738-52 pubmed publisher
Ma Q, Zhao J, Cao W, Liu J, Cui S. Estradiol decreases taurine level by reducing cysteine sulfinic acid decarboxylase via the estrogen receptor-α in female mice liver. Am J Physiol Gastrointest Liver Physiol. 2015;308:G277-86 pubmed publisher
product information
CatalogNo :
12601-1-AP
AntigenName :
GCLC
Package :
150UL
Price :
299 USD
Exsists20ul :
20ul trial size available
FullName :
glutamate-cysteine ligase, catalytic subunit
Immunogen :
Recombinant Protein
Species :
human, mouse, rat
Host :
Rabbit
IsConjugated :
Unconjugated
AntigenSpecies :
human
Application :
WB, IP, IHC, ELISA
Clonlity :
Polyclonal
IsoType :
IgG
Synonyms :
Gamma ECS, GCLC, GCS, GCS heavy chain, GLCL, GLCLC
PrimaryOrSecondary :
Primary
AntibodyBuffer :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
GenBankNo :
BC022487
Category :
Binding Proteins;Metabolism;
PurifyMethod :
Antigen affinity purification
NewAb :
False
IsSellable :
True
AppTiter :
IHC 1:200 ; IP 1:500 ; WB 1:5000 ;
company information
Proteintech Group
2201 W. Campbell Park Dr. STE12
Chicago, IL 60612
Proteintech@ptglab.com
https://www.ptglab.com
1-312-455-8498
headquarters: USA
At Proteintech, we produce every single antibody we sell; we do not rely on or supply to any other antibody providers: our products are unique and we are 100% accountable for each one. We realize this accountability by validating in-house, providing extensive technical support and guaranteeing your success: in addition to helping you troubleshoot your experiment, we will offer you a full cash refund if you are in any way dissatisfied. We can guarantee satisfaction because we have confidence in our products, confidence cultivated by the science behind our antibodies: we make them using as much of the native protein as possible, and purifying them using affinity purification with the original antigen. We carry out antibody production over a 102-day period, which allows for better antigen fitting to MHC molecules and affinity maturation in the host. This approach results in higher affinity antibodies with greater sensitivity, which you can use in any application and in multiple species.
You can only buy Proteintech antibodies directly from Proteintech or via one of its approved distributors — when you receive your antibody and see the Proteintech logo on the vial, know that you hold something that is truly unique.