product summary
company name :
Protein Laboratories Rehovot
product type :
protein
product name :
Horse leptin
catalog :
LEP-4
more info or order :
product information
Title :
Horse leptin
Description :
Recombinant horse leptin is an one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Recombinant horse leptin was purified by proprietary chromatographic techniques, (unpublished).
Source :
E. coli
Physical Appearance :
White lyophilized (freeze-dried) powder.
Formulation :
The recombinant protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Solubility :
It is recommended to reconstitute the lyophilized recombinant horse leptin in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability :
Lyophilized recombinant horse leptin although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 recombinant horse leptin mg/ml and up to 2 mg and filter sterilization recombinant horse leptin can be stored at +4C.
Purity :
The purity of recombinant horse leptin is greater than 99.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.
Amino Acid Sequence :
The sequence of the first five N-terminal amino acids of recombinant horse leptin was determined and was found to be Ala-Val-Pro-Ile-Gln
Dimers and Aggregates :
The purified racombinant horse leptin (16K) consists of > 97% monomers as determined by gel-filtration chromatography.
Biological Activity :
PLR’s recombinant horse leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Endotoxin :
The endotoxin content of recombinant horse leptin is less than 0.1 ng/µg (IEU/µg) of horse leptin
Protein content :
Protein quantitation of recombinant horse leptin was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.20 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Usage :
This recombinant horse leptin is offered by PLR for laboratory research
more info or order :
company information
Protein Laboratories Rehovot