product summary
company name :
Protein Laboratories Rehovot
product type :
protein
product name :
Rat leptin
catalog :
LEP-12
more info or order :
product information
Title :
Rat leptin
Description :
Recombinant rat leptin, (rLEP)is a single polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, rLEP was purified by proprietary chromatographic techniques, according to Salomon et al (2006) Protein Expression and Purifcation 47, 128–136.
Source :
E. coli
Physical Appearance :
White lyophilized (freeze-dried) powder.
Formulation :
The recombinant rat leptin was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Solubility :
It is recommended to reconstitute the lyophilized recombinant rat leptin in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml. Recombinant rat leptin can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Stability :
Lyophilized recombinant rat leptin although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 mg/ml of recombinant rat leptin and up to 2 mg/ml and filter sterilization recombinant rat leptin can be stored at +4C.
Purity :
The purity of recombinant rat leptin is greater than 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel. (c) RP-chromatography
Amino Acid Sequence :
The sequence of the first five N-terminal amino acids of recombinant rat leptin was determined and was found to be Ala-Val-Pro-Ile-His.
Dimers and Aggregates :
The purified recombinant rat leptin (16K) consists of > 99% monomers as determined by gel-filtration chromatography.
Biological Activity :
PLR’s recombinant rat leptin is fully biologically active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Endotoxin :
Less than 0.1 ng/µg (IEU/µg) of recombinant rat leptin
Protein content :
Protein quantitation of recombinant rat leptin was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.20 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Usage :
The recombinant rat leptin is offered by PLR for laboratory research
more info or order :
company information
Protein Laboratories Rehovot
related products
browse more products
questions and comments