product summary
company name :
Protein Laboratories Rehovot
product type :
protein
product name :
Caprine placental lactogen
catalog :
GHP-19
more info or order :
product information
Title :
Caprine placental lactogen
Description :
Caprine recombinant placental lactogen, one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques (see Sakal et al. J. Endocrinology (1998), 159, 509-518). Caprine recombinant placental lactogen is also capable of activating human and other heterologous growth receptors but not ruminat growth hormone receptors.
Source :
E. coli
Physical Appearance :
White lyophilized (freeze-dried) powder.
Formulation :
The caprine recombinant placental lactogen was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO3.
Solubility :
It is recommended to reconstitute the lyophilized caprine recombinant placental lactogen in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Stability :
Lyophilized caprine recombinant placental lactogen although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution of caprine recombinant placental lactogen at > 0.1 mg/ml and up to 4 mg/ml and filter sterilization caprine recombinant placental lactogen can be stored at 4C for several weeks.
Purity :
The purity of caprine recombinant placental lactogen is greater than 99.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.
Amino Acid Sequence :
The sequence of the first four N-terminal amino acids of caprine recombinant placental lactogen was determined and was found to be Ala-Glu-Asn-Tyr.
Dimers and Aggregates :
The purified caprine recombinant placental lactogen (23K) consists of > 99% monomers as determined by gel-filtration chromatography.
Biological Activity :
PLR’s caprine recombinant placental lactogen is fully biologically active as evidenced by inducing proliferation of Nb2 cells.
Endotoxin :
Less than 0.1 ng/µg (IEU/µg) of caprine recombinant placental lactogen
Protein content :
Protein quantitation of caprine recombinant placental lactogen was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Usage :
The caprine recombinant placental lactogen is offered by PLR for laboratory research
more info or order :
company information
Protein Laboratories Rehovot