protein

Human growth hormone soluble receptor (so called hGH binding protein)

ECD-1

Recombinant hGHBP, one polypeptide chain containing 248 amino acids and having a molecular mass of ~ 28 kDa, hGHBP was purified by proprietary chromatographic techniques, (see Gertler et al. J Biol Chem. (1996) 271, 24482-91).

Escherichia coli

White lyophilized (freeze-dried) powder.

The protein was lyophilized from a concentrated (0.3 mg/ml) solution in Tris-HCl, pH 8 + 300 mM NaHCO3 and 10-fold excess (w/w)of glycine.

It is recommended to reconstitute the lyophilized hGHBP in sterile 0.4% NaHCO3 adjusted to pH 8 or in ditilled water, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized hGHBP although stable at room temperature for 3 weeks, should be stored desiccated below -18?C. Upon reconstitution at > 0.1 hGHBP mg/ml and filter sterilization hGHBP can be stored at 4?C for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Greater than 95.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Ser-Gly-Ser.

The purified hGHBP (28K) consists of > 98% monomers as determined by gel-filtration chromatography.

PLR’s hGHBP is fully biologically active as evidenced by its ability of forming 2:1 complex with hGH.

Less than 0.1 ng/µg (IEU/µg) of hGHBP (28 K).

Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 2.64 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

This material is offered by PLR for laboratory research