protein

Rat insulin-like growth factor 1 (rIGF-1) CYT-103

CYT-103

IGF-1 is a small protein secreted mainly but not solely by the liver and circulating in blood mostly as a complex with various IGF binding proteins in the blood. It has growth-regulating, insulin-like, and mitogenic activities and it is secreted in response to growth hormone stimulation. Recombinant rat IGF-I produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 7.6 kDa.

E. coli

White powder

The protein was lyophilized with no additives.

It is recommended to reconstitute the lyophilized rIGF-I in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized rIGF-I although stable at room temperature for several weeks, should be stored desiccated below -18 C. Upon reconstitution at > 0.1 rIGF-I mg/ml and up to 2 mg/ml and filter sterilization rbIGF-I can be stored at +4C.

Greater than 98.0% as determined by SDS-PAGE.

MGPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKSA.

The purified rIGF-I consists of > 95% monomers as determined by gel-filtration chromatography.

The ED50, was calculated 2 methods: 1. Stimulation of protein synthesis in rat L6 myoblasts ED50 was found to be < 30ng/ml corresponding to a Specific Activity of 33,334IU/mg. 2. Type 1 IGF receptor binding assay ED50 was found to be <10ng/ml corresponding to a Specific Activity of 100,000IU/mg.

Endotoxin level is <0.5 EU/ug

Protein quantification was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.68 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNAman computer analysis program of protein sequences.

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