FGF 2 Human | ProSpec CYT-218 product information

product summary

company name :

ProSpec

product type :

protein

product name :

FGF 2 Human

catalog :

CYT-218

quantity :

10 ug

price :

50 USD

more info or order :

ProSpec product webpage

citations: 5

Reference
Ahn S, Lee H, Lee J, Yoon H, Chun W, Kim G. A novel cell-printing method and its application to hepatogenic differentiation of human adipose stem cell-embedded mesh structures. Sci Rep. 2015;5:13427 pubmed publisher
Hsieh Y, Intawicha P, Lee K, Chiu Y, Lo N, Ju J. LIF and FGF cooperatively support stemness of rabbit embryonic stem cells derived from parthenogenetically activated embryos. Cell Reprogram. 2011;13:241-55 pubmed publisher
Intawicha P, Wang S, Hsieh Y, Lo N, Lee K, Huang S, et al. Proteomic profiling of rabbit embryonic stem cells derived from parthenotes and fertilized embryos. PLoS ONE. 2013;8:e67772 pubmed publisher
Miyasaka E, Raghavan S, Gilmont R, Mittal K, Somara S, Bitar K, et al. In vivo growth of a bioengineered internal anal sphincter: comparison of growth factors for optimization of growth and survival. Pediatr Surg Int. 2011;27:137-43 pubmed publisher
Higuchi A, Shen P, Zhao J, Chen C, Ling Q, Chen H, et al. Osteoblast differentiation of amniotic fluid-derived stem cells irradiated with visible light. Tissue Eng Part A. 2011;17:2593-602 pubmed publisher

product information

Short Name :

FGF 2 Human

Full Name :

Fibroblast Growth Factor-Basic Human Recombinant

BarCode :

CYT-218

Level1 :

10 ug

Price1 :

50 USD

Level2 :

50ug

Price2 :

130

Level3 :

1mg

Price3 :

1080

Category :

Growth Factors

Sub Category :

Fibroblast Growth Factor

LinkUrl :

P09038

Amino acid sequence :

MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLPMSAKS.

Biological Activity :

The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is 0.5 ng/ml, corresponding to a specific activity of 2,000,000 Units/mg.

Description :

Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17353 Dalton. The FGF-b is purified by proprietary chromatographic techniques.

Formulation :

The protein was lyophilized from a concentrated (1mg/ml) solution in PBS, pH 7.4.

Physical Appearance :

Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content :

Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Fibroblast Growth Factor-b as a Reference Standard.

Purity :

Greater than 96.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Solubility :

It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile 18MM-VM->)-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source :

Escherichia Coli

Stability :

Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution FGF-b should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Synonyms :

Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.

more info or order :

ProSpec product webpage