IGF1 Human | ProSpec CYT-216 product information

product summary

company name :

ProSpec

product type :

protein

product name :

IGF1 Human

catalog :

CYT-216

quantity :

20 ug

price :

50 USD

more info or order :

ProSpec product webpage

citations: 1

Reference
Zhao F, Han J, Chen X, Wang J, Wang X, Sun J, et al. miR-223 enhances the sensitivity of non-small cell lung cancer cells to erlotinib by targeting the insulin-like growth factor-1 receptor. Int J Mol Med. 2016;38:183-91 pubmed publisher

product information

Short Name :

IGF1 Human

Full Name :

Insulin-Like Growth Factor 1 Human Recombinant

BarCode :

CYT-216

Level1 :

20 ug

Price1 :

50 USD

Level2 :

100ug

Price2 :

130

Level3 :

1mg

Price3 :

270

Category :

Growth Factors

Sub Category :

Insulin-Like Growth Factor

LinkUrl :

P01343

Amino acid sequence :

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA.

Biological Activity :

The activity as determined by the dose-dependent proliferation of mouse FDC-P1 is less than 1.0 ng/ml, corresponding to a specific activity of 1x10 6 units/mg.

Description :

Insulin-Like Growth Factor-I Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.7kDa. IGF-I is purified by proprietary chromatographic techniques.

Formulation :

The protein was lyophilized without any additives.

Physical Appearance :

Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content :

Protein quantitation was carried out by two independent methods: . 1. UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IGF1 as a Reference Standard.

Purity :

Greater than 98.0% as determined by:. (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

References :

1. Title :Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis.

Solubility :

It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MM-VM->)-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source :

Escherichia Coli

Stability :

Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IGF1 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Synonyms :

Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.

more info or order :

ProSpec product webpage