APRIL Antibody [Sacha-2] | ProSci XA-1025 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProSci

product type :

antibody

product name :

APRIL Antibody [Sacha-2]

catalog :

XA-1025

quantity :

0.1 mg

price :

330 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

Sacha-2

The same clone is also sold as:

Enzo Life Sciences: ALX-804-804-C100
Abnova: MAB3365

reactivity :

human, mouse

application :

immunocytochemistry, FACS

product information

Catalog Number :

XA-1025

Product Name :

APRIL Antibody [Sacha-2]

Host Species :

Rat

Clonality :

Monoclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.1 mg

List Price :

330 USD

Clone :

Sacha-2

Isotype :

IgG2a

CrossReactivity :

Human, Mouse

ALTnames :

APRIL, TALL2, ZTNF2, UNQ383/PRO715, Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand, APRIL, CD256, TALL-2, TRDL-1

Immunogen :

APRIL (monoclonal Sacha-2) antibody was raised against recombinant human APRIL (aa. 105-250).

Purity :

APRIL (monoclonal Sacha-2) antibody was purified from concentrated hybridoma tissue culture supernatant. APRIL (monoclonal Sacha-2) antibody was purified to 95% in SDS-PAGE.

Buffer :

PBS containing 0.02% sodium azide

Uses :

E, ICC, FACS

Application Summary :

APRIL antibody can be used to recognize human and mouse APRIL by ELISA (capture), Flow Cytometry, and Immunocytochemistry. Method: HEK-293T cells expressing surface membrane associated human APRIL (aa 92-233) or a mock control were injected in chicken liver and frozen in OCT. 8 m sections of injected frozen liver were fixed in acetone, washed in PBS and incubated with Sacha-2 at 10 g/mL for 1hr after blocking in PBS/1%BSA. Human APRIL was revealed with 3-amino-9-Ethyl-carbazole (AEC) following 45 min. incubation with goat anti-rat HRP. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mouse APRIL (thick line). Cells were incubated on ice for 30 min in 50 l FACS buffer (PBS, 5% Fetal calf serum, 0.02% sodium azide) containing 1 g/mL of Sacha-2 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry. This product is for research use only.

Storage :

APRIL antibody can be stored at 4 C, stable for one year.