RIP3 Antibody [Rippy-3] | ProSci XA-1023 product information

product summary

company name :

ProSci

product type :

antibody

product name :

RIP3 Antibody [Rippy-3]

catalog :

XA-1023

quantity :

0.1 mg

price :

330 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Rippy-3

reactivity :

human

application :

western blot

more info or order :

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citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 3a	LaRocca T, Stivison E, Mal-Sarkar T, Hooven T, Hod E, Spitalnik S, et al. CD59 signaling and membrane pores drive Syk-dependent erythrocyte necroptosis. Cell Death Dis. 2015;6:e1773 pubmed publisher

product information

Catalog Number :

XA-1023

Product Name :

RIP3 Antibody [Rippy-3]

Host Species :

Mouse

Clonality :

Monoclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.1 mg

List Price :

330 USD

Clone :

Rippy-3

Isotype :

IgG1

CrossReactivity :

Human

ALTnames :

RIP3RIP-like protein kinase 3, RIP-3, RIP3

Immunogen :

RIP3 (monoclonal Rippy-3) antibody was raised against recombinant human RIP3.

Purity :

RIP3 (monoclonal Rippy-3) antibody was purified to >=95% in SDS-PAGE.

Buffer :

PBS containing 0.02% sodium azide

Uses :

Application Summary :

RIP3 antibody can be used to recognize human RIP3 by western blot. Method: HEK 293T cells (5x105) mock transfected (lane 1) or transfected (lane 2) with an expression plasmid coding for human RIP3 were harvested, lysed and cell extracts (5x105) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the Rippy-3 monoclonal antibody at a 1:1000 dilution for 2 hours. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG and a chemiluminescence detection system. This product is for research use only.

Storage :

RIP3 antibody can be stored at 4 C, stable for one year.

General References :

Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB: P.W. Yu, et al.; Curr. Biol. 9, 539 (1999).

General References :

RIP3, a novel apoptosis-inducing kinase: X. Sun, et al.; J. Biol. Chem. 274, 16871 (1999).

General References :

Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-kappaB: N.J. Pazdernik, et al.; Mol. Cell. Biol. 19, 6500 (1999).

General References :

RIP4 (DIK/PKK), a novel member of the RIP kinase family, activates NF-kappa B and is processed during apoptosis: E. Meylan, et al.; EMBO Rep. 3, 1201 (2002).

more info or order :

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