ORAI1 Antibody [6D11A11] | ProSci PM-5207 product information

product summary

company name :

ProSci

product type :

antibody

product name :

ORAI1 Antibody [6D11A11]

catalog :

PM-5207

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6D11A11

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

ProSci product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:500; tbl 1 western blot; mouse; 1:1000; tbl 1	Liu J, Xin L, Benson V, Allen D, Ju Y. Store-operated calcium entry and the localization of STIM1 and Orai1 proteins in isolated mouse sinoatrial node cells. Front Physiol. 2015;6:69 pubmed publisher

product information

Catalog Number :

PM-5207

Product Name :

ORAI1 Antibody [6D11A11]

Host Species :

Mouse

Clonality :

Monoclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Clone :

6D11A11

Isotype :

IgG2b

CrossReactivity :

Human, Mouse, Rat

Background :

ORAI1 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one fo rm of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

ALTnames :

ORAI1 Antibody [6D11A11] : IMD9, ORAT1, CRACM1, TMEM142A

Immunogen :

Mouse monoclonal ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.

Purity :

ORAI1 Monoclonal Antibody is immunoaffinity chromotography purified IgG.

Buffer :

ORAI1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

ORAI1 monoclonal antibody can be stored at -20 C, stable for one year.

General References :

Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.

General References :

Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.

General References :

Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.

more info or order :

ProSci product webpage