ORAI1 Antibody [3F6H5] | ProSci PM-5205 product information

product summary

company name :

ProSci

product type :

antibody

product name :

ORAI1 Antibody [3F6H5]

catalog :

PM-5205

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3F6H5

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

ProSci product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1b	Kondratska K, Kondratskyi A, Yassine M, Lemonnier L, Lepage G, Morabito A, et al. Orai1 and STIM1 mediate SOCE and contribute to apoptotic resistance of pancreatic adenocarcinoma. Biochim Biophys Acta. 2014;1843:2263-9 pubmed publisher

product information

Catalog Number :

PM-5205

Product Name :

ORAI1 Antibody [3F6H5]

Host Species :

Mouse

Clonality :

Monoclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Clone :

3F6H5

Isotype :

IgG1

CrossReactivity :

Human, Mouse, Rat

Background :

ORAI1 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one fo rm of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

ALTnames :

ORAI1 Antibody [3F6H5] : IMD9, ORAT1, CRACM1, TMEM142A

Immunogen :

Mouse monoclonal ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.

Specificity :

ORAI1 antibody is predicted to have no cross reactivity to other members in the ORAI family.

Purity :

ORAI1 Monoclonal Antibody is immunoaffinity chromotography purified IgG.

Buffer :

ORAI1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

ORAI1 monoclonal antibody can be stored at -20 C, stable for one year.

General References :

Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.

General References :

Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.

General References :

Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.

more info or order :

ProSci product webpage