ORAI3 Antibody [2H2G9] | ProSci PM-4911 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProSci

product type :

antibody

product name :

ORAI3 Antibody [2H2G9]

catalog :

PM-4911

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2H2G9

The same clone is also sold as:

Rockland Immunochemicals: 200-301-DH4
OriGene: TA320075
Abnova: MAB8084
Invitrogen: MA5-15778

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

product information

Catalog Number :

PM-4911

Product Name :

ORAI3 Antibody [2H2G9]

Host Species :

Mouse

Clonality :

Monoclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Clone :

2H2G9

Isotype :

IgG2a

CrossReactivity :

Human, Rat

Background :

ORAI3 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3.

ALTnames :

ORAI3 Antibody [2H2G9] : TMEM142C

Immunogen :

A 19 amino acid synthetic peptide from near the carboxy terminus of human ORAI3.

Purity :

ORAI3 Monoclonal Antibody is immunoaffinity chromotography purified IgG.

Buffer :

ORAI3 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

ORAI3 antibody can be used for detection of ORAI3 by Western blot at 2 g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 g/mL. For immunofluorescence start at 5 g/mL.

Storage :

ORAI3 monoclonal antibody can be stored at -20 C, stable for one year.

General References :

Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.

General References :

Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.

General References :

Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.

General References :

Mercer JC, DeHaven WI, Smyth JT, et al. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 2006; 281:24979-90.