This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
ProSci
product type :
antibody
product name :
Apaf-1 Antibody [5E11]
catalog :
PM-2016
quantity :
0.02 mg, 0.1 mg
price :
79, 315 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5E11

The same clone is also sold as:
reactivity :
human, mouse, rat
application :
immunohistochemistry
product information
Catalog Number :
PM-2016
Product Name :
Apaf-1 Antibody [5E11]
Host Species :
Mouse
Clonality :
Monoclonal
Conjugation :
Unconjugated
Product Type :
Primary Antibody
Size :
0.02 mg, 0.1 mg
List Price :
79, 315 USD
Clone :
5E11
CrossReactivity :
Human, Mouse, Rat
Background :
Apaf-1 Monoclonal Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.
ALTnames :
Apaf-1 Antibody [5E11] : CED4, APAF-1
Immunogen :
A peptide corresponding to amino acids near the carboxy terminus of human Apaf1.
Purity :
Apaf-1 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Buffer :
Apaf-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration :
1 mg/mL
Uses :
E, IHC
Application Summary :
Apaf1 antibody can be used for detection of Apaf1 by immunohistochemistry at 0.5 mg/mL. Apaf1 has not been confirmed for use in Western Blot.
Storage :
Apaf-1 monoclonal antibody can be stored at -20 C, stable for one year.
General References :
Zou H, Henzel WJ, Liu X, et al. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997; 90:405-13.
General References :
Cecconi F, Alvarez-Bolado G, Meyer BI, et al. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998; 94:727-37.
General References :
Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997; 91:479-89.
General References :
Hu Y, Benedict MA, Wu D, et al. Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation. Proc. Natl. Acad. Sci. USA 1998; 95:4386-91.
company information
ProSci
12170 Flint Place
Poway, CA 92064
orders@prosci-inc.com
www.prosci-inc.com
1-888-513-9525
headquarters: USA