ATG4A Antibody | ProSci 8071 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProSci

product type :

antibody

product name :

ATG4A Antibody

catalog :

8071

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry

product information

Catalog Number :

8071

Product Name :

ATG4A Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4A, also known as AUTL2, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4).

ALTnames :

Autophagy related 4A cysteine peptidase, APG4-A, APG4-A, AUTL2

Immunogen :

ATG4A antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ATG4A.

Specificity :

ATG4A antibody is human, mouse and rat reactive. At least two isoforms of ATG4A are known to exist; this antibody will detect both isoforms. ATG4A is predicted to not cross-react with other ATG4 proteins.

Purity :

ATG4A antibody is affinity chromatography purified via peptide column.

Buffer :

ATG4A antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC

Application Summary :

ATG4A antibody can be used for detection of ATG4A by Western blot at 1 - 2 g/ml. Antibody can also be used for immunohistochemistry starting at 5 g/mL.

Storage :

ATG4A antibody can be stored at 4 C for three months and -20 C, stable for up to one year.

General References :

Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906.

General References :

Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.

General References :

Marino G, Uria JA, Puente XS, et al. Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy. J. Biol. Chem. 2003; 278:3671-8.

General References :

Li M, Hou Y, Wang J, et al. Kinetic comparisons of mammalian Atg4 homologues indicate selective preferences towards diverse Atg8 substrates. J. Biol. Chem. 2011; 286:7327-38.