Beclin 2 Antibody | ProSci 7989 product information

product summary

company name :

ProSci

product type :

antibody

product name :

Beclin 2 Antibody

catalog :

7989

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

ProSci product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 4 western blot; mouse; fig 4	Kuramoto K, Wang N, Fan Y, Zhang W, Schoenen F, Frankowski K, et al. Autophagy activation by novel inducers prevents BECN2-mediated drug tolerance to cannabinoids. Autophagy. 2016;12:1460-71 pubmed publisher

product information

Catalog Number :

7989

Product Name :

Beclin 2 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human

Background :

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells (1,2). Beclin 2 is a mammalian specific homolog of the autophagy protein Beclin 1 (3). Like Beclin 1, Beclin 2 interacts with Bcl-2 and class III PI3K complex components. However, Beclin 2 functions in an additional lysosomal degradation pathway and is required for ligand-induced endolysosomal degradation of several G protein-coupled receptors (3). Beclin 2 is also required for agonist-induced lysosome-mediated degradation of EGFR in lung cancer cells, suggesting that it may also play a role in regulating other intracellular signaling pathways (4).

ALTnames :

BECN2, BECN1L1, Beclin-1-like protein 1, Beclin 1 autophagy related pseudogene 1, BECN1P1

Immunogen :

Beclin 2 antibody was raised against a 16 amino acid peptide near the amino terminus of human Beclin 2.

Specificity :

Beclin 2 antibody is human specific. At least two isoforms of Beclin 2 are known to exist; this antibody will only detect the longer isoform. Beclin 2 antibody is predicted to not cross-react with Beclin 1.

Purity :

Beclin 2 antibody is affinity chromatography purified via peptide column.

Buffer :

Beclin 2 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

Beclin 2 antibody can be used for detection of Beclin 2 by Western blot at 1 - 2 g/ml. Antibody can also be used for immunohistochemistry starting at 5 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

Beclin 2 antibody can be stored at 4 C for three months and -20 C, stable for up to one year.

General References :

Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.

General References :

Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.

General References :

He C, Wei Y, Sun K, et al. Beclin 2 functions in autophagy, degradation of G protein-coupled receptors, and metabolism. Cell 2013; 154:1085-99.

General References :

Zhang W and He C. Regulation of plasma membrane receptors by a new autophagy-related BECN/Beclin family member. Autophagy 2014; 10: epub.

more info or order :

ProSci product webpage