NOX1 Antibody | ProSci 7921 product information

product summary

company name :

ProSci

product type :

antibody

product name :

NOX1 Antibody

catalog :

7921

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

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citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1e	Jafari N, Kim H, Park R, Li L, Jang M, Morris A, et al. CRISPR-Cas9 Mediated NOX4 Knockout Inhibits Cell Proliferation and Invasion in HeLa Cells. PLoS ONE. 2017;12:e0170327 pubmed publisher

product information

Catalog Number :

7921

Product Name :

NOX1 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human

Background :

Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress (1). NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox (1). Three splice variants of NOX1 have been identified, NOH-1L, NOH-1S and NOH-1Lv (2). NOH-1S is a voltage-gated proton channel that participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain (1-3). NOX1 have the potential to be effective treatments for a range of ischemic diseases (4).

ALTnames :

NOX1 Antibody: MOX1, NOH1, NOH-1, GP91-2, MOX1, NADPH oxidase 1, Mitogenic oxidase 1, NOX-1

Immunogen :

NOX1 antibody was raised against a 15 amino acid peptide near the center of human NOX1.

Specificity :

NOX1 antibody is human specific.

Purity :

NOX1 antibody is affinity chromatography purified via peptide column.

Buffer :

NOX1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

NOX1 antibody can be used for detection of NOX1 by Western blot at 1 - 2 g/ml. Antibody can also be used for Immunohistochemistry starting at 5 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

NOX1 antibody can be stored at 4 C for three months and -20 C, stable for up to one year.

General References :

Helmcke I, Heumuller S, Tikkanen R, et al. Identification of structural elements in Nox1 and Nox4 controlling localization and activity. Antioxid. Redox Signal. 2009; 11:1279-87.

General References :

Piccoli C, D'Aprile A, Ripoli M, et al. Bone-marrow derived hematopoietic stem/progenitor cells express multiple isoforms of NADPH oxidase and produce constitutively reactive oxygen species. Biochem. Biophys. Res. Commun. 2007; 353:965-72.

General References :

Lee JG, Lim EJ, Park DW, et al. A combination of Lox-1 and Nox1 regulates TLR9-mediated foam cell formation. Cell Signal. 2008; 20:2266-75.

General References :

Stanic B, Katsuyama M, and Miller FJ Jr. An oxidized extracellular oxidation-reduction state increases Nox1 expression and proliferation in vascular smooth muscle cells via epidermal growth factor receptor activation. Arterioscler. Thromb. Vasc. Biol. 2010; 30:2234-41.

more info or order :

ProSci product webpage