Alpha-tubulin Antibody | ProSci 7599 product information

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product summary

company name :

ProSci

product type :

antibody

product name :

Alpha-tubulin Antibody

catalog :

7599

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

product information

Catalog Number :

7599

Product Name :

Alpha-tubulin Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.

ALTnames :

Alpha-tubulin Antibody: TUBA2, bA408E5.3, TUBA2, TUBA3D, Alpha-tubulin 2

Immunogen :

Alpha-tubulin antibody was raised against an 18 amino acid peptide near the amino terminus of human alpha-tubulin

Specificity :

Alpha-tubulin antibody is human, mouse and rat reactive.

Purity :

Alpha-tubulin antibody is affinity chromatography purified via peptide column.

Buffer :

Alpha-tubulin antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 0.25 - 0.5 g/ml. Antibody can also be used for Immunohistochemistry starting at 5 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

Alpha-tubulin antibody can be stored at 4 C for three months and -20 C, stable for up to one year.

General References :

McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci. 2001; 114:2723-33.

General References :

Barra HA, Arce CA, and Argarana CE. Posttranslational tyrosination/detyrosination of tubulin. Mol. Neurobiol. 1988; 2:133-53.

General References :

Fukshima N, Furuta D, Hidaka Y, et al. Post-translational modifcations of tubulin in the nervous system. J. Neurochem. 2009; 109:683-693.