THEM4 Antibody | ProSci 5227 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProSci

product type :

antibody

product name :

THEM4 Antibody

catalog :

5227

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

product information

Catalog Number :

5227

Product Name :

THEM4 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

THEM4 Antibody: THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.

ALTnames :

THEM4 Antibody: CTMP, CTMP, Acyl-coenzyme A thioesterase THEM4, Carboxyl-terminal modulator protein, Acyl-CoA thioesterase THEM4

Immunogen :

THEM4 antibody was raised against an 18 amino acid synthetic peptide near the center of human THEM4.

Specificity :

The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.

Purity :

THEM4 Antibody is affinity chromatography purified via peptide column.

Buffer :

THEM4 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

THEM4 antibody can be used for detection of THEM4 by Western blot at 1 - 2 g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

THEM4 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Maira SM, Galetic I, Brazil DP, et al. Carboxyl-terminal modulator protein (CTMP), a negative regulator of PKB/Akt and v-Akt at the plasma membrane. Science2001; 294:374-80.

General References :

Knobbe CB, Reifberger J, Blaschke B, et al. Hypermethylation and transcriptional downregulation of the carboxyl-terminal modulator protein gene in glioblastomas. J. Natl. Cancer Inst.2004; 96:483-6.

General References :

Zhao H, Martin BM, Bisoffi M, et al. The Akt C-terminal modulator protein is an acyl-CoA thioesterase of the hotdog-fold family. Biochemistry2009; 48:5507-9.

General References :

Parcellier A, Tintignac LA, Zhuravleva E, et al. Carboxy-terminal modulator protein (CTMP) is a mitochondrial protein that sensitizes cells to apoptosis. Cell Signal.2009; 21:639-50.