Collagen I Antibody | ProSci 49-396 product information

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product summary

company name :

ProSci

product type :

antibody

product name :

Collagen I Antibody

catalog :

49-396

quantity :

0.05 mg

price :

350 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

product information

Catalog Number :

49-396

Product Name :

Collagen I Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.05 mg

List Price :

350 USD

CrossReactivity :

Bovine, Human, Mouse, Rat

Background :

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.

ALTnames :

Alpha-1 type I collagenOI4

Immunogen :

Collagen I antibody was raised against Collagen Type I from human and bovine placenta (Human).

Purity :

Immunoaffinity Chromatography

Buffer :

0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA;, pH 8.0, 0.01% sodium azide.

Uses :

E, WB, IHC, IP

Application Summary :

Collagen I antibody can be used in ELISA, and immunohistochemistry starting at 1:50.

Storage :

Store Collagen I antibody at 4 C or -20 C. As with all antibodies avoid freeze/thaw cycles.