Ambra1 Antibody | ProSci 4557 product information

product summary

company name :

ProSci

product type :

antibody

product name :

Ambra1 Antibody

catalog :

4557

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

ProSci product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:100	Sepe S, Nardacci R, Fanelli F, Rosso P, Bernardi C, Cecconi F, et al. Expression of Ambra1 in mouse brain during physiological and Alzheimer type aging. Neurobiol Aging. 2014;35:96-108 pubmed publisher
	Nardacci R, Amendola A, Ciccosanti F, Corazzari M, Esposito V, Vlassi C, et al. Autophagy plays an important role in the containment of HIV-1 in nonprogressor-infected patients. Autophagy. 2014;10:1167-78 pubmed publisher

product information

Catalog Number :

4557

Product Name :

Ambra1 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse

Background :

Ambra1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.

ALTnames :

Ambra1 Antibody: DCAF3, WDR94, KIAA1736, Activating molecule in BECN1-regulated autophagy protein 1

Immunogen :

Ambra1 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human Ambra1.

Purity :

Ambra1 Antibody is affinity chromatography purified via peptide column.

Buffer :

Ambra1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

Ambra1 antibody can be used for the detection of Ambra1 by Western blot at 2 g/mL. Antibody can also be used for immunohistochemistry starting at 5 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

Ambra1 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906.

General References :

Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis1993; 14:2501-5.

General References :

Liang XH, Jackson S, Seaman M, et al. Induction of autophagy and inhibition of tumorigenesis by beclin 1. Nature1999; 402:672-6.

General References :

Fimia GM, Stoykova A, Romagnoli A, et al. Ambra1 regulates autophagy and development of the nervous system. Nature2007; 447:1121-5.

more info or order :

ProSci product webpage