TEM4 Antibody | ProSci 4367 product information

product summary

company name :

ProSci

product type :

antibody

product name :

TEM4 Antibody

catalog :

4367

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

ProSci product webpage

citations: 1

Reference
Mitin N, Rossman K, Currin R, Anne S, Marshall T, Bear J, et al. The RhoGEF TEM4 Regulates Endothelial Cell Migration by Suppressing Actomyosin Contractility. PLoS ONE. 2013;8:e66260 pubmed publisher

product information

Catalog Number :

4367

Product Name :

TEM4 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human

Background :

TEM4 Antibody: Rho GTPases, which are activated by specific guanine-nucleotide exchange factors (GEFs), play pivotal roles in several cellular functions. TEM4, encoding a protein containing 1510 amino acids, contains a RhoGEF-specific Dbl homology (DH) domain but lacks their typical pleckstrin homology domain. TEM4 is a Rho-specific GEF with novel structural and regulatory properties and predominant expression in the heart. It couples tyrosine kinase signals with the activation of the rho/rac GTPases, thus leading to cell differentiation and/or proliferation. Elevated levels of TEM4, TEM5, TEM6, TEM7 and TEM7R were also raised in breast cancer tissues. TEM4 could also prove to be useful targets therapeutically.

ALTnames :

TEM4 Antibody: TEM4, P164RHOGEF, p164-RhoGEF, KIAA0337, TEM4, Rho guanine nucleotide exchange factor 17, 164 kDa Rho-specific guanine-nucleotide exchange factor

Immunogen :

TEM4 antibody was raised against a 18 amino acid synthetic peptide near the center of human TEM4.

Purity :

TEM4 Antibody is affinity chromatography purified via peptide column.

Buffer :

TEM4 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

TEM4 antibody can be used for detection of TEM4 by Western blot at 1 - 2 g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

TEM4 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Ruemenapp U, Freichel-Blomquist A, Wittinghofer B, et al. A mammalian Rho-specific guanine-nucleotide exchange factor (p164-RhoGEF) without a pleckstrin homology domain. Biochem. J.2002; 366:721-8.

General References :

Carson-Walter EB, Watkins DN, Nanda A, et al. Cell surface tumor endothelial markers are conserved in mice and humans. Cancer Res.2001; 61:6649-55.

General References :

Davies G, Cunnick GH, Mansel RE, et al. Levels of expression of endothelial markers specific to tumour-associated endothelial cells and their correlation with prognosis in patients with breast cancer. Clinical & Experimental Metastasis2004; 21:31-7.

General References :

Nanda A and St Croix B. Tumor endothelial markers: new targets for cancer therapy. Curr. Opin. Oncol.2004; 16:44-9.

more info or order :

ProSci product webpage