BRCC36 Antibody | ProSci 4331 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProSci

product type :

antibody

product name :

BRCC36 Antibody

catalog :

4331

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

product information

Catalog Number :

4331

Product Name :

BRCC36 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

BRCC36 Antibody: Together with the breast and ovarian predisposition proteins BRCA1 and BRCA2 and RAD51 and BRCC45, BRCC36 forms a holoenzyme complex that possesses a ubiquitin E3 ligase activity. Aberrant levels of BRCC36 were detected in sporadic breast tumors and depletion of either BRCC36 or BRCC45 by siRNA resulted in increased sensitivity to ionizing radiation and defects in the G2/M checkpoint, indicating that BRCC36 acts to enhance cellular survival following DNA damage. Recent experiments have shown that BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation, suggesting that BRCC36 may be an important target in the treatment of radiation-resistant breast tumors. At least two isoforms of BRCC36 are known to exist.

ALTnames :

BRCC36 Antibody: C6.1A, BRCC36, CXorf53, C6.1A, Lys-63-specific deubiquitinase BRCC36, BRCA1-A complex subunit BRCC36

Immunogen :

BRCC36 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC36.

Purity :

BRCC36 Antibody is affinity chromatography purified via peptide column.

Buffer :

BRCC36 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

BRCC36 antibody can be used for detection of BRCC36 by Western blot at 0.5 - 2 g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

BRCC36 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Dong Y, Hakimi MA, Chen X, et al. Regulation of BRCC, a holoenzyme complex containing BRCA1 and BRCA2, by a signalsome-like subunit and its role in DNA repair. Mol. Cell2003; 12:1087-99.

General References :

Chen X, Arciero CA, Wang C, et al. BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation. Cancer Res.2006; 66:5039-46.