LIS1 Antibody | ProSci 4287 product information

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product summary

company name :

ProSci

product type :

antibody

product name :

LIS1 Antibody

catalog :

4287

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry

product information

Catalog Number :

4287

Product Name :

LIS1 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

LIS1 Antibody: Lissencephaly is a severe brain developmental disease characterized by the mislocalization of cortical neurons, a smooth cerebral surface, mental retardation, and seizures. Classical lissencephaly is caused by sporadic mutations in the LIS1 gene. While LIS1 is known to act in a pathway deactivating the lipid messenger platelet-activating factor, LIS1 forms a complex with Nudel and 14-3-3epsilon which is then transported from neuronal cell bodies through the actions of DISC1 and KIF5A, a microtubule-dependent directed motor protein kinesin. Decreased expression of LIS1 blocked neural stem cell division, morphogenesis, and motility, suggesting that LIS1 plays an important role in neuronal cell proliferation and localization in the developing brain. At least two isoforms of LIS1 are known to exist.

ALTnames :

LIS1 Antibody: MDS, LIS1, LIS2, MDCR, PAFAH, MDS, PAFAHA, Platelet-activating factor acetylhydrolase IB subunit alpha, Lissencephaly-1 protein, LIS-1

Immunogen :

LIS1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human LIS1.

Purity :

LIS1 Antibody is affinity chromatography purified via peptide column.

Buffer :

LIS1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, ICC, IF

Application Summary :

LIS1 antibody can be used for detection of LIS1 by Western blot at 0.5 - 1 g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

LIS1 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

McManus MF and Golden JA. Neuronal migration in developmental disorders. J. Child Neurol.2005; 20:280-6.

General References :

Reiner O, Carrozzo R, Shen Y, et al. Isolation of a Miller-Dieker lissencephaly gene containing G protein b-subunit-like repeats. Nature1993; 364:717-21.

General References :

Hattori M, Adachi H, Tsujimoto M, et al. Miller-Dieker lissencephaly gene encodes a subunit of brain platelet activating factor. Nature1994; 370:216-8.

General References :

Taya S, Shinoda T, Tsuboi D, et al. DISC1 regulates the transport of the NUDEL/LIS1/14-3-3e complex through kinesin-1. J. Neurosci.2007; 27:15-26.