ProSci

antibody

TDP43 Antibody

4285

0.02 mg, 0.1 mg

79, 315 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunocytochemistry

4285

TDP43 Antibody

Rabbit

Polyclonal

Unconjugated

Primary Antibody

0.02 mg, 0.1 mg

79, 315 USD

IgG

Human, Mouse, Rat

TDP43 Antibody: TDP43 was initially identified as a novel cellular protein that bound to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3'splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.

TDP43 Antibody: ALS10, TDP-43, TDP43, TAR DNA-binding protein 43

TDP43 antibody was raised against a 18 amino acid synthetic peptide from near the center of human TDP43.

At least two isoforms are known to exist for this protein; this TDP43 antibody only recongizes the longer of the two isoforms.

TDP43 Antibody is affinity chromatography purified via peptide column.

TDP43 Antibody is supplied in PBS containing 0.02% sodium azide.

1 mg/mL

E, WB, ICC, IF

TDP43 antibody can be used for detection of TDP43 by Western blot at 0.5 - 2 g/mL. Antibody can also be used for immunocytochemistry starting at 5 g/mL. For immunofluorescence start at 20 g/mL.

TDP43 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Ou SH, Wu F, Garcia-Martinez LF, et al. Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs. J. Virol.1995; 69:3584-96.

Buratti E, Dork T, Zuccato E, et al. Nuclear factor TDP-43 and SR proteins promote in vitro and in vivo CFTR exon 9 skipping. EMBO J.2001; 20:1774-84.

Neumann M, Sampathu DM, Kwong LK, et al. Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Science2006; 314:42-3.