ORAI1 Antibody | ProSci 4281 product information

product summary

company name :

ProSci

product type :

antibody

product name :

ORAI1 Antibody

catalog :

4281

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

ProSci product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; rat	Ferretti R, Marques M, Khurana T, Santo Neto H. Expression of calcium-buffering proteins in rat intrinsic laryngeal muscles. Physiol Rep. 2015;3: pubmed publisher
western blot; rat; 1:1000; fig 4	Garcia Alvarez G, Lu B, Yap K, Wong L, Thevathasan J, Lim L, et al. STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs. Mol Biol Cell. 2015;26:1141-59 pubmed publisher
western blot; rat; 1:500	Saliba Y, Keck M, Marchand A, Atassi F, OuillÃ© A, Cazorla O, et al. Emergence of Orai3 activity during cardiac hypertrophy. Cardiovasc Res. 2015;105:248-59 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:50	Cross B, Hack A, Reinhardt T, Rao R. SPCA2 regulates Orai1 trafficking and store independent Ca2+ entry in a model of lactation. PLoS ONE. 2013;8:e67348 pubmed publisher
immunohistochemistry - frozen section; rat; 3 ug/ml western blot; rat; 1 ug/ml	Wolkowicz P, Huang J, Umeda P, Sharifov O, TABENGWA E, Halloran B, et al. Pharmacological evidence for Orai channel activation as a source of cardiac abnormal automaticity. Eur J Pharmacol. 2011;668:208-16 pubmed publisher
western blot; rat; 1:1000	Gemes G, Bangaru M, Wu H, Tang Q, Weihrauch D, Koopmeiners A, et al. Store-operated Ca2+ entry in sensory neurons: functional role and the effect of painful nerve injury. J Neurosci. 2011;31:3536-49 pubmed publisher
	Collins H, He L, Zou L, Qu J, Zhou L, Litovsky S, et al. Stromal interaction molecule 1 is essential for normal cardiac homeostasis through modulation of ER and mitochondrial function. Am J Physiol Heart Circ Physiol. 2014;306:H1231-9 pubmed publisher
	Wang P, Umeda P, Sharifov O, Halloran B, TABENGWA E, Grenett H, et al. Evidence that 2-aminoethoxydiphenyl borate provokes fibrillation in perfused rat hearts via voltage-independent calcium channels. Eur J Pharmacol. 2012;681:60-7 pubmed publisher
	Edwards J, Murphy R, Cully T, von Wegner F, Friedrich O, Launikonis B. Ultra-rapid activation and deactivation of store-operated Ca(2+) entry in skeletal muscle. Cell Calcium. 2010;47:458-67 pubmed publisher

product information

Catalog Number :

4281

Product Name :

ORAI1 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse

Background :

ORAI1 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor.

ALTnames :

ORAI1 Antibody: IMD9, ORAT1, CRACM1, TMEM142A, Calcium release-activated calcium channel protein 1, Protein orai-1

Immunogen :

ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.

Specificity :

This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

Purity :

ORAI1 Antibody is affinity chromatography purified via peptide column.

Buffer :

ORAI1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC

Application Summary :

ORAI1 antibody can be used for detection of ORAI1 by Western blot at 0.5 - 2 g/mL. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 g/mL.

Storage :

ORAI1 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol.2001; 19:497-521.

General References :

Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature2006; 441:179-85.

General References :

Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta.2006; 1763:1161-8.

more info or order :

ProSci product webpage