Bub1 Antibody | ProSci 4229 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProSci

product type :

antibody

product name :

Bub1 Antibody

catalog :

4229

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry

product information

Catalog Number :

4229

Product Name :

Bub1 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse

Background :

Bub1 Antibody: Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.

ALTnames :

Bub1 Antibody: BUB1A, BUB1L, hBUB1, BUB1A

Immunogen :

Bub1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1.

Purity :

Bub1 Antibody is affinity chromatography purified via peptide column.

Buffer :

Bub1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, ICC, IF

Application Summary :

Bub1 antibody can be used for detection of bub1 by Western blot at 2 - 4 g/mL. Antibody can also be used for immunocytochemistry starting at 10 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

Bub1 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Pangilinan F, Li Q, Weaver T, et al. Mammalian BUB1 protein kinases: map positions and in vivo expression. Genomics1997; 46:379-88.

General References :

Williams GL, Roberts TM and Gjoerup OV. Bub1: Escapades in a cellular world. Cell Cycle2007; 6:1699-704.

General References :

Meraldi P and Sorger PK. A dual role for Bub1 in the spindle checkpoint and chromosome congression. EMBO J.2005; 24:1621-33.

General References :

Tang Z, Shu H, Oncel D, et al. Phosphorylation of cdc20 by bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint. Mol. Cell2004; 16:387-97.