StrepII-tag Antibody | ProSci 4217 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ProSci

product type :

antibody

product name :

StrepII-tag Antibody

catalog :

4217

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

application :

western blot, ELISA

product information

Catalog Number :

4217

Product Name :

StrepII-tag Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Tag

Background :

StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.

ALTnames :

StrepII-tag Antibody:

Immunogen :

StrepII-tag antibody was raised against the amino acid sequence "gggWSHPQFEK".

Purity :

StrepII-tag Antibody is affinity chromatography purified via peptide column.

Buffer :

StrepII-tag Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB

Application Summary :

StrepII-tag antibody can be used for the detection of StrepII by Western blot at 1 g/mL.

Storage :

StrepII-tag antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Schmidt TGM and Skerra A. The random peptide-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng.1993; 6:109-22.

General References :

Schmidt TGM, Koepe J, Frank R, et al. Molecular interactions between the Strep-tag affinity peptide and its cognate target, streptavidin. J. Mol. Biol.1996; 255:753-6.