ARF-BP1 Antibody | ProSci 4213 product information

product summary

company name :

ProSci

product type :

antibody

product name :

ARF-BP1 Antibody

catalog :

4213

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

more info or order :

ProSci product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; human western blot; human	Lee C, Yang Y, Chen C, Liu J. Syk-mediated tyrosine phosphorylation of mule promotes TNF-induced JNK activation and cell death. Oncogene. 2016;35:1988-95 pubmed publisher
western blot; human	Khoronenkova S, Dianov G. USP7S-dependent inactivation of Mule regulates DNA damage signalling and repair. Nucleic Acids Res. 2013;41:1750-6 pubmed publisher

product information

Catalog Number :

4213

Product Name :

ARF-BP1 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

ARF-BP1 Antibody: The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.

ALTnames :

ARF-BP1 Antibody: MULE, Ib772, LASU1, UREB1, HECTH9, URE-B1, ARF-BP1, HSPC272, KIAA0312, KIAA1578, E3 ubiquitin-protein ligase HUWE1, ARF-binding protein 1

Immunogen :

ARF-BP1 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human ARF-BP1.

Purity :

ARF-BP1 Antibody is affinity chromatography purified via peptide column.

Buffer :

ARF-BP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, ICC, IF

Application Summary :

ARF-BP1 antibody can be used for detection of ARF-BP1 by Western blot at 1 g/mL. Antibody can also be used for immunocytochemistry starting at 5 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

ARF-BP1 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Gallagher SJ, Kefford RF and Rizos H. The ARF tumour suppressor. Int. J. Biochem. Cell Biol.2006; 38:1637-41.

General References :

Pomerantz J, Schreiber-Agus N, Leigeois NJ, et al. The Ink4a tumor suppressor gene product, p19ARF interacts with MDM2 and neutralizes MDM2’s inhibition of p53. Cell1998; 92:725-34.

General References :

Chen D, Kon N, Li M, et al. ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor. Cell2005; 121:1071-83.

General References :

Zhong Q, Gao W, Du F, et al. Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis. Cell2005; 121:1085-95.

more info or order :

ProSci product webpage