ProSci

antibody

STIM2 Antibody

4125

0.02 mg, 0.1 mg

79, 315 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, immunocytochemistry

4125

STIM2 Antibody

Rabbit

Polyclonal

Unconjugated

Primary Antibody

0.02 mg, 0.1 mg

79, 315 USD

IgG

Human, Mouse, Rat

STIM2 Antibody: In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least three isoforms of STIM2 are known to exist. This STIM2 antibody is predicted to have no cross-reactivity to STIM1.

STIM2 Antibody: KIAA1482, Stromal interaction molecule 2

STIM2 antibody was raised against a 17 amino acid synthetic peptide from near the center of human STIM2.

STIM2 Antibody is affinity chromatography purified via peptide column.

STIM2 Antibody is supplied in PBS containing 0.02% sodium azide.

1 mg/mL

E, WB, IHC, IF

STIM2 antibody can be used for detection of STIM2 by Western blot at 0.5 - 2 g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 g/mL. For immunofluorescence start at 20 g/mL.

STIM2 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Luik RM and Lewis RS. New insights into the molecular mechanisms of store-operated Ca2+ signaling in T cells. Trends Mol. Med.2007; 13:103-7.

Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature2006; 441:179-85.

Zhang SL, Yu Y, Roos J, et al. STIM1 is a Ca 2+ sensor that activates CRAC channels and migrates from the Ca 2+ store to the plasma membrane. Nature2005; 437:902-5.

Spassova MA, Soboloff J, He L-P, et al. STIM1 has a plasma membrane role in the activation of store-operated Ca2+ channels. Proc. Natl. Acad. Sci. USA2006; 103:4040-5.