STIM2 Antibody | ProSci 4123 product information

product summary

company name :

ProSci

product type :

antibody

product name :

STIM2 Antibody

catalog :

4123

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

ProSci product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:400; tbl 1 western blot; mouse; 1:1000; tbl 1	Liu J, Xin L, Benson V, Allen D, Ju Y. Store-operated calcium entry and the localization of STIM1 and Orai1 proteins in isolated mouse sinoatrial node cells. Front Physiol. 2015;6:69 pubmed publisher
western blot; rat; 1:1000	Steinbeck J, Henke N, Opatz J, Gruszczynska-Biegala J, Schneider L, Theiss S, et al. Store-operated calcium entry modulates neuronal network activity in a model of chronic epilepsy. Exp Neurol. 2011;232:185-94 pubmed publisher
western blot; human western blot; mouse	Schuhmann M, Stegner D, Berna-Erro A, Bittner S, Braun A, Kleinschnitz C, et al. Stromal interaction molecules 1 and 2 are key regulators of autoreactive T cell activation in murine autoimmune central nervous system inflammation. J Immunol. 2010;184:1536-42 pubmed publisher
	Lefievre L, Nash K, Mansell S, Costello S, Punt E, Correia J, et al. 2-APB-potentiated channels amplify CatSper-induced Ca(2+) signals in human sperm. Biochem J. 2012;448:189-200 pubmed publisher

product information

Catalog Number :

4123

Product Name :

STIM2 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

STIM2 Antibody: In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least three isoforms of STIM2 are known to exist. This STIM2 antibody is predicted to have no cross-reactivity to STIM1.

ALTnames :

STIM2 Antibody: KIAA1482, Stromal interaction molecule 2

Immunogen :

STIM2 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human STIM2.

Purity :

STIM2 Antibody is affinity chromatography purified via peptide column.

Buffer :

STIM2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

STIM2 antibody can be used for detection of STIM2 by Western blot at 0.5 - 1 g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

STIM2 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Luik RM and Lewis RS. New insights into the molecular mechanisms of store-operated Ca 2+ signaling in T cells. Trends Mol. Med.2007; 13:103-7.

General References :

Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature2006; 441:179-85.

General References :

Zhang SL, Yu Y, Roos J, et al. STIM1 is a Ca 2+ sensor that activates CRAC channels and migrates from the Ca 2+ store to the plasma membrane. Nature2005; 437:902-5.

General References :

Spassova MA, Soboloff J, He L-P, et al. STIM1 has a plasma membrane role in the activation of store-operated Ca2+ channels. Proc. Natl. Acad. Sci. USA2006; 103:4040-5.

more info or order :

ProSci product webpage