STIM1 Antibody | ProSci 4119 product information

product summary

company name :

ProSci

product type :

antibody

product name :

STIM1 Antibody

catalog :

4119

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - frozen section

more info or order :

ProSci product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:500; fig s1	Garcia-Alvarez G, Lu B, Yap K, Wong L, Thevathasan J, Lim L, et al. STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs. Mol Biol Cell. 2015;26:1141-59 pubmed publisher
western blot; mouse; 1:1000	Edwards J, Murphy R, Cully T, von Wegner F, Friedrich O, Launikonis B. Ultra-rapid activation and deactivation of store-operated Ca(2+) entry in skeletal muscle. Cell Calcium. 2010;47:458-67 pubmed publisher
immunohistochemistry - frozen section; rat; 1:1500 western blot; rat; 1:500	Gemes G, Bangaru M, Wu H, Tang Q, Weihrauch D, Koopmeiners A, et al. Store-operated Ca2+ entry in sensory neurons: functional role and the effect of painful nerve injury. J Neurosci. 2011;31:3536-49 pubmed publisher
	Garcia-Alvarez G, Shetty M, Lu B, Yap K, Oh-hora M, Sajikumar S, et al. Impaired spatial memory and enhanced long-term potentiation in mice with forebrain-specific ablation of the Stim genes. Front Behav Neurosci. 2015;9:180 pubmed publisher
	Lefievre L, Nash K, Mansell S, Costello S, Punt E, Correia J, et al. 2-APB-potentiated channels amplify CatSper-induced Ca(2+) signals in human sperm. Biochem J. 2012;448:189-200 pubmed publisher

product information

Catalog Number :

4119

Product Name :

STIM1 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

STIM1 Antibody: In T lymphocytes, the sole pathway for Ca 2+ entry following antigen-receptor binding is through store-operated Ca 2+ -release-activated Ca 2+ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca 2+ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca 2+ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca 2+ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca 2+ entry.

ALTnames :

STIM1 Antibody: GOK, TAM, IMD10, D11S4896E, GOK, Stromal interaction molecule 1

Immunogen :

STIM1 antibody was raised against a 24 amino acid synthetic peptide from near the carboxy terminus of human STIM1.

Specificity :

At least two isoforms of STIM1 are known to exist; this antibody will detect only the larger form. This STIM1 antibody is predicted to have no cross-reactivity to STIM2.

Purity :

STIM1 Antibody is affinity chromatography purified via peptide column.

Buffer :

STIM1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC

Application Summary :

STIM1 antibody can be used for detection of STIM1 by Western blot at 1 - 2 g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 g/mL.

Storage :

STIM1 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Luik RM and Lewis RS. New insights into the molecular mechanisms of store-operated Ca 2+ signaling in T cells. Trends Mol. Med. 2007; 13:103-7.

General References :

Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.

General References :

Zhang SL, Yu Y, Roos J, et al. STIM1 is a Ca 2+ sensor that activates CRAC channels and migrates from the Ca 2+ store to the plasma membrane. Nature 2005; 437:902-5.

General References :

Spassova MA, Soboloff J, He L-P, et al. STIM1 has a plasma membrane role in the activation of store-operated Ca2+ channels. Proc. Natl. Acad. Sci. USA 2006; 103:4040-5.

more info or order :

ProSci product webpage