ORAI3 Antibody | ProSci 4117 product information

product summary

company name :

ProSci

product type :

antibody

product name :

ORAI3 Antibody

catalog :

4117

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

ProSci product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:500; fig 1e	Zeng B, Chen G, Garcia Vaz E, Bhandari S, Daskoulidou N, Berglund L, et al. ORAI channels are critical for receptor-mediated endocytosis of albumin. Nat Commun. 2017;8:1920 pubmed publisher
immunocytochemistry; mouse; 1:200; tbl 1 western blot; mouse; 1:1000; tbl 1	Liu J, Xin L, Benson V, Allen D, Ju Y. Store-operated calcium entry and the localization of STIM1 and Orai1 proteins in isolated mouse sinoatrial node cells. Front Physiol. 2015;6:69 pubmed publisher
western blot; rat; 1:500	Saliba Y, Keck M, Marchand A, Atassi F, OuillÃ© A, Cazorla O, et al. Emergence of Orai3 activity during cardiac hypertrophy. Cardiovasc Res. 2015;105:248-59 pubmed publisher
immunohistochemistry - frozen section; rat; 3 ug/ml western blot; rat; 1 ug/ml	Wolkowicz P, Huang J, Umeda P, Sharifov O, TABENGWA E, Halloran B, et al. Pharmacological evidence for Orai channel activation as a source of cardiac abnormal automaticity. Eur J Pharmacol. 2011;668:208-16 pubmed publisher
	Wang P, Umeda P, Sharifov O, Halloran B, TABENGWA E, Grenett H, et al. Evidence that 2-aminoethoxydiphenyl borate provokes fibrillation in perfused rat hearts via voltage-independent calcium channels. Eur J Pharmacol. 2012;681:60-7 pubmed publisher

product information

Catalog Number :

4117

Product Name :

ORAI3 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

ORAI3 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter.

ALTnames :

ORAI3 Antibody: TMEM142C, TMEM142C, Protein orai-3, Transmembrane protein 142C

Immunogen :

ORAI3 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human ORAI3.

Specificity :

This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2.

Purity :

ORAI3 Antibody is affinity chromatography purified via peptide column.

Buffer :

ORAI3 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, ICC

Application Summary :

ORAI3 antibody can be used for detection of ORAI3 by Western blot at 1 - 4 g/mL. Antibody can also be used for immunocytochemistry starting at 10 g/mL.

Storage :

ORAI3 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.

General References :

Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.

General References :

Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.

General References :

Mercer JC, DeHaven WI, Smyth JT, et al. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 2006; 281:24979-90.

more info or order :

ProSci product webpage