TIGAR Antibody | ProSci 4051 product information

product summary

company name :

ProSci

product type :

antibody

product name :

TIGAR Antibody

catalog :

4051

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

ProSci product webpage

citations: 1

Reference
Meng L, Kohn K, Pommier Y. Dose-response transition from cell cycle arrest to apoptosis with selective degradation of Mdm2 and p21WAF1/CIP1 in response to the novel anticancer agent, aminoflavone (NSC 686,288). Oncogene. 2007;26:4806-16 pubmed

product information

Catalog Number :

4051

Product Name :

TIGAR Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse

Background :

TIGAR Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death; loss of its functions contributes to the development of most cancers. Recent studies have demonstrated the ability of p53 to regulate the expression of several proteins involved in glycolysis and oxidative phosphorylation, such as TIGAR, SCO2, and phosphoglycerate mutase. TIGAR is a recently discovered protein that functions to regulate glycolysis and protect cells against oxidative stress. TIGAR is similar in structure to proteins in the phosphoglycerate mutase family, most notably 6-phosphofructo-2-kinase, suggesting TIGAR may function as a fructose bisphosphatase. Expression of TIGAR in transfected cells correlated with an inhibition of glycolysis and decreased levels of reactive oxygen species and p53-induced apoptosis, indicating that TIGAR may act to modulate the apoptotic response to p53, thereby allowing cells to survive mild or transient stresses.

ALTnames :

TIGAR Antibody: FR2BP, TIGAR, C12orf5, Fructose-2, 6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator

Immunogen :

TIGAR antibody was raised against a 19 amino acid synthetic peptide from near the center of human TIGAR.

Purity :

TIGAR Antibody is affinity chromatography purified via peptide column.

Buffer :

TIGAR Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

TIGAR antibody can be used for detection of TIGAR by Western blot at 0.5 - 2 g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

TIGAR antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Guimaraes DP and Hainaut P. TP53: a key gene in human cancer. Biochimie2002; 84:83-93.

General References :

Corcoran CA, Huang Y, and Sheikh MS. The regulation of energy generating pathways by p53. Cancer Biol. Ther.2006; 5:1610-3.

General References :

Bensaad K, Tsuruta A, Selak MA, et al. TIGAR, a p53-inducible regulator of glycolysis and apoptosis. Cell2006; 126:107-20.

more info or order :

ProSci product webpage