ORAI1 Antibody | ProSci 4041 product information

product summary

company name :

ProSci

product type :

antibody

product name :

ORAI1 Antibody

catalog :

4041

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

ProSci product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig s2	Albarrán L, Lopez J, Amor N, Martin Cano F, Berna Erro A, Smani T, et al. Dynamic interaction of SARAF with STIM1 and Orai1 to modulate store-operated calcium entry. Sci Rep. 2016;6:24452 pubmed publisher
immunocytochemistry; human western blot; human	Huang Y, Chang S, Harn H, Huang H, Lin H, Shen M, et al. Mechanosensitive store-operated calcium entry regulates the formation of cell polarity. J Cell Physiol. 2015;230:2086-97 pubmed publisher
	Kuras Z, Yun Y, Chimote A, Neumeier L, Conforti L. KCa3.1 and TRPM7 channels at the uropod regulate migration of activated human T cells. PLoS ONE. 2012;7:e43859 pubmed publisher
	Lefievre L, Nash K, Mansell S, Costello S, Punt E, Correia J, et al. 2-APB-potentiated channels amplify CatSper-induced Ca(2+) signals in human sperm. Biochem J. 2012;448:189-200 pubmed publisher
	Edwards J, Murphy R, Cully T, von Wegner F, Friedrich O, Launikonis B. Ultra-rapid activation and deactivation of store-operated Ca(2+) entry in skeletal muscle. Cell Calcium. 2010;47:458-67 pubmed publisher
	Bisaillon J, Motiani R, Gonzalez Cobos J, Potier M, Halligan K, Alzawahra W, et al. Essential role for STIM1/Orai1-mediated calcium influx in PDGF-induced smooth muscle migration. Am J Physiol Cell Physiol. 2010;298:C993-1005 pubmed publisher

product information

Catalog Number :

4041

Product Name :

ORAI1 Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse

Background :

ORAI1 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor.

ALTnames :

ORAI1 Antibody: IMD9, ORAT1, CRACM1, TMEM142A, Calcium release-activated calcium channel protein 1, Protein orai-1

Immunogen :

ORAI1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human ORAI1.

Specificity :

This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.

Purity :

ORAI1 Antibody is affinity chromatography purified via peptide column.

Buffer :

ORAI1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 g/mL. Antibody can also be used for immunohistochemistry starting at 10 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

ORAI1 antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.

General References :

Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.

General References :

Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.

more info or order :

ProSci product webpage