DRAM Antibody | ProSci 4033 product information

product summary

company name :

ProSci

product type :

antibody

product name :

DRAM Antibody

catalog :

4033

quantity :

0.02 mg, 0.1 mg

price :

79, 315 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

ProSci product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 0.5 ug/ml	Mehta S, Lin Y, Chen W, Yu F, Cao L, He Q, et al. Manganese Superoxide Dismutase Deficiency Exacerbates Ischemic Brain Damage Under Hyperglycemic Conditions by Altering Autophagy. Transl Stroke Res. 2011;2:42-50 pubmed
western blot; mouse	Walls K, Ghosh A, Franklin A, Klocke B, Ballestas M, Shacka J, et al. Lysosome dysfunction triggers Atg7-dependent neural apoptosis. J Biol Chem. 2010;285:10497-507 pubmed publisher

product information

Catalog Number :

4033

Product Name :

DRAM Antibody

Host Species :

Rabbit

Clonality :

Polyclonal

Conjugation :

Unconjugated

Product Type :

Primary Antibody

Size :

0.02 mg, 0.1 mg

List Price :

79, 315 USD

Isotype :

IgG

CrossReactivity :

Human, Mouse, Rat

Background :

DRAM Antibody: Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.

ALTnames :

DRAM Antibody: DRAM, DRAM, DNA damage-regulated autophagy modulator protein 1, Damage-regulated autophagy modulator

Immunogen :

DRAM antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human DRAM.

Purity :

DRAM Antibody is affinity chromatography purified via peptide column.

Buffer :

DRAM Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration :

1 mg/mL

Uses :

E, WB, IHC, IF

Application Summary :

DRAM antibody can be used for detection of DRAM by Western blot at 0.5 - 1 g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 g/mL. For immunofluorescence start at 20 g/mL.

Storage :

DRAM antibody can be stored at 4 C for three months and -20 C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

General References :

Crighton D, Wilkinson S, O’Prey J, et al. DRAM, a p53-induced modulator of autophagy, is critical for apoptosis. Cell 2006; 126:121-34.

General References :

Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.

General References :

Crighton D, Wilkinson S, and Ryan KM. DRAM links autophagy to p53 and programmed cell death. Autophagy 2007; 3:72-4. (07-01D)

more info or order :

ProSci product webpage